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Table 1 List of CPH substrates made and used in the study along with substrate sources

From: A new generation of versatile chromogenic substrates for high-throughput analysis of biomass-degrading enzymes

Substrate

Source

CPH-2-hydroxyethylcellulose (CPH-HE cellulose)

N/A

CPH-amylopectin

Potato

CPH-amylose

Potato

CPH-arabinan

Sugar beet

CPH-arabinoxylan

Wheat

CPH-casein

Bovine milk

CPH-curdlan

Alcaligenes faecalis

CPH-dextran

Leuconostoc spp.

CPH-galactomannan

Carob

CPH-laminarin

Laminaria digitata

CPH-lichenan

Icelandic moss

CPH-pachyman

Poria cocos

CPH-pectic galactan

Potato

CPH-pullulan

Aureobasidium pullulans

CPH-rhamnogalacturonan I

Potato

CPH-rhamnogalacturonan I (-Gal)a

Potato

CPH-rhamnogalacturonan

Soy bean

CPH-xylan

Beechwood

CPH-xyloglucan

Tamarind

CPH-β-glucan from barley

Barley

CPH-β-glucan from oat

Oat

CPH-β-glucan from yeast

Yeast

ICB-Arabidopsis

Rosette leaves from Arabidopsis thaliana Col-0 (adult plant)

ICB-bagasse

Saccharum officinarum (dried adult plant, stem and leaves)

ICB-fenugreek seeds

Trigonella spp. seeds

ICB-hemp

Cannabis spp. (dried adult plant, stem and leaves)

ICB-lupin seeds

Lupinus angustifolius seeds

ICB-lupin seeds

Lupinus angustifolius seeds

ICB-tobacco

Leaves from Nicotiana benthamiana (young plant)

ICB-wheat straw

Triticum spp. (dried adult plant, stem and leaves)

ICB-willow

Salix spp. (dried adult plant, milled

  1. a(β-1,4-D-galactan side chains removed with endo-galactanase gal). N/A, not applicable.