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Table 1 Changes in the fatty acid composition of strain CM4A*

From: pH-induced change in cell susceptibility to butanol in a high butanol-tolerant bacterium, Enterococcus faecalis strain CM4A

 

No solvents

2.0% butanol

 

pH 6.0

pH 6.8

pH 8.0

pH 10.0

pH 6.0

pH 6.8

pH 8.0

pH 10.0

C14:0

4.2 ± 0.5

4.6 ± 1.3

6.5 ± 1.9

6.9 ± 0.7

5.8 ± 0.3

5.0 ± 1.9

5.8 ± 0.1

4.6 ± 1.9

C14:1ω7c

0.1 ± 0.2

0.2 ± 0.2

0.5 ± 0.2

0.6 ± 0.1

0.3 ± 0.3

0.3 ± 0.3

0.6 ± 0.1

0.2 ± 0.2

C16:0

41.5 ± 1.1

41.6 ± 1.0

39.6 ± 3.3

39.2 ± 2.9

49.4 ± 4.1

44.8 ± 1.8

41.0 ± 1.9

43.5 ± 1.7

C16:1

0.8 ± 0.7

1.2 ± 1.1

0.9 ± 0.9

0.9 ± 0.9

1.0 ± 1.0

1.9 ± 2.0

1.3 ± 1.5

0.8 ± 0.7

C16:1ω7c

4.4 ± 1.3

6.1 ± 2.0

6.5 ± 0.8

7.5 ± 1.1

6.4 ± 1.2

7.2 ± 2.6

7.5 ± 1.8

6.9 ± 4.4

C18:0

2.2 ± 0.6

1.5 ± 0.2

1.3 ± 0.5

0.9 ± 0.2

2.7 ± 1.2

1.6 ± 0.6

1.2 ± 0.6

5.1 ± 3.6

C18:1ω7c

46.3 ± 2.4

44.9 ± 4.8

44.3 ± 3.0

43.8 ± 1.7

30.4 ± 0.9

37.6 ± 4.9

42.0 ± 4.1

38.6 ± 2.8

cyclo-C19:0

0.6 ± 0.8

ND

0.4 ± 0.7

ND

3.5 ± 1.1

1.3 ± 0.6

0.7 ± 0.2

0.3 ± 0.3

C20:1

0.0 ± 0.1

ND

0.1 ± 0.1

ND

0.5 ± 0.9

0.1 ± 0.2

ND

ND

Total C14

4.3 ± 0.5

4.8 ± 1.5

6.9 ± 2.1

7.5 ± 0.8

6.1 ± 0.6

5.4 ± 2.3

6.4 ± 0.1

4.8 ± 1.8

Total saturated fatty acids

47.9 ± 0.6

47.6 ± 1.6

47.4 ± 4.7

47.1 ± 3.3

57.9 ± 4.7

51.5 ± 1.6

47.9 ± 1.4

53.2 ± 1.8

  1. *Each fatty acid composition is described as a percentage of the whole-cell lipids. The values are the means ± standard deviations of three independent measurements. The double-bond positions of C16:1 and C20:1 were not identified. ‘Total saturated fatty acids’ is the sum of myristic acid (C14:0), palmitic acid (C16:0), and stearic acid (C18:0). Abbreviations: C14:1ω7c, cis-tetradecenoic acid; C16:1ω7c, palmitoleic acid; C18:1ω7c, cis-vaccenic acid; cyclo-C19:0, cis-11,12-methylene octadecanoic acid; ND, not detected. The italicized values are discussed in the text.