Figure 4From: Analysis of Clostridium beijerinckii NCIMB 8052’s transcriptional response to ferulic acid and its application to enhance the strain toleranceConstruction of pSAAT-ptb_Gro. The final shuttle vector was designed so that the expression of groES-groEL is under the control of the ptb promoter and so that it has two origins of replication and antibiotic resistances for use within both E. coli and Clostridium. The plasmids are drawn with directions of the relevant genes and the restriction sites used in each step of construction.Back to article page