Table 3 Bacterial strains and plasmids used in this study

Strains

C. beijerinckii NCIMB 8052

Wild type

C. acetobutylicum ATCC 824

Wild type

E. coli DH5α

fhuA2 Δ (argF-lacZ) U169 phoA glnV44 Φ80 Δ (lacZ) M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Plasmids

pDEWMCS

Modified pDEW201 plasmid, MCS contains restriction sites for NotI, XhoI, BamHI, XmaI, XbaI, EcoRV, and KpnI; Amp^R

pSA12

Em^r, shuttle vector, ColE1 ori, pIM13 oriII

15

pSAAT

Em^R, Amp^R, shuttle vector, ColE1 ori, pIM13 oriII, T1-4

This study

pSAAT-ptb

Em^R, Amp^R, shuttle vector, ColE1 ori, pIM13 oriII, T1-4, Pptb,

This study

pSAAT-ptb_Gro

Em^R, Amp^R, shuttle vector, ColE1 ori, pIM13 oriII, T1-4, Pptb-groES-groEL

This study