New generation biofuel: continuous acidogenesis of sucrose-raffinose mixture simulating vinasse is promoted by γ-alumina pellets

Lappa, Katerina; Kandylis, Panagiotis; Bastas, Nikolaos; Klaoudatos, Stavros; Athanasopoulos, Nikolaos; Bekatorou, Argyro; Kanellaki, Maria; Koutinas, Athanasios A

doi:10.1186/s13068-015-0255-6

Biotechnology for Biofuels and Bioproducts

Table 2 Continuous acidogenesis of synthetic media using γ-alumina-supported anaerobic mixed culture

From: New generation biofuel: continuous acidogenesis of sucrose-raffinose mixture simulating vinasse is promoted by γ-alumina pellets

Influent				Effluent
Feed	Initial sugar (g/L)	Flow rate (L/day)	Operation time (days)	pH	Ethanol (ml/L)	Acetic acid (g/L)	Lactic acid (g/L)	Succinic acid (g/L)	Malic acid (g/L)	Butyric acid (g/L)	Total VFAs (g/L)	Residual sugar (g/L)	Sugar conversion (%)	VFA yield (g/g)	VFA productivity (g/L/day)
Sucrose	20	0.2	1	4.9	7.7	0.18	3.83	1.32	1.01	-	6.34				2.54
	20	0.2	2	4.8	8.9	0.21	4.21	1.36	1.01	-	6.79	5.44	72.8	0.47	2.72
	20	0.4	3	4.7	10.5	0.23	4.41	1.34	1.08	-	7.06				5.65
	20	0.4	4	4.8	5.7	0.33	3.89	1.01	1.01	-	6.24				4.99
	20	0.4	6	4.8	2.9	0.39	3.35	0.84	1.01	-	5.59	7.06	64.7	0.43	4.47
	20	0.3	7	4.7	1.1	0.38	3.32	0.91	1.00	-	5.61				3.37
	20	0.3	8	4.7	1.0	0.35	3.83	0.99	0.99	-	6.16				3.70
	20	0.3	9	4.7	1.0	0.34	4.60	1.09	0.99	-	7.02	7.60	62.0	0.57	4.21
	20	0.25	10	4.7	1.94	0.32	5.35	1.17	0.97	-	7.81				3.91
	20	0.25	11	4.6	1.96	0.35	6.61	1.26	0.97	-	9.18				4.59
	20	0.25	12	4.5	1.85	0.32	6.91	1.29	0.95	-	9.48	4.24	78.8	0.60	4.74
	20	0.25	13	4.6	2.11	0.40	7.59	1.34	0.97	-	10.29				5.15
	20	0.25	14	4.6	1.85	0.38	7.37	1.34	0.96	-	10.05				5.03
	20	0.25	15	4.5	1.75	0.38	8.16	1.47	0.96	-	12.96	6.88	65.6	0.99	6.48
	20	0.25	16	4.7	2.18	0.34	6.58	1.30	0.96	-	9.19				4.60
	20	0.25	17	4.7	2.33	0.38	6.70	1.34	0.98	-	9.39				4.70
	20	0.25	18	4.7	2.4	0.38	7.02	1.40	0.77	-	9.57	6.99	65.0	0.74	4.79
	20	0.25	21	4.7	2.28	0.37	6.19	1.38	0.99	-	8.93				4.47
	20	0.2	23	4.5	4.38	0.37	9.04	2.30	0.97	-	12.67	5.06	74.7	0.85	5.07
	20	0.2	24	4.6	3.46	0.41	7.51	2.59	0.99	-	11.50				4.60
	20	0.2	25	4.6	3.43	0.42	6.10	2.61	1.01	-	10.15				4.06
	20	0.2	26	4.6	2.96	0.42	5.82	2.58	1.01	-	9.83	8.12	59.4	0.83	3.93
	20	0.2	27	4.6	6.04	0.43	5.99	2.54	1.02	-	9.98				3.99
	20	0.2	28	4.6	5.78	0.38	5.67	2.34	1.02	-	9.41				3.76
	20	0.2	29	4.6	5.26	0.36	5.61	2.24	1.02	-	9.23	7.74	61.3	0.75	3.69
	20	0.2	31	4.7	4.28	0.37	5.50	2.12	1.02	-	9.01				3.60
	20	0.2	32	4.7	1.89	0.33	5.62	2.29	1.03	-	9.28				3.71
	20	0.2	33	4.7	1.27	0.36	5.57	2.37	1.03	-	9.33	7.67	61.6	0.76	3.73
	20	0.2	34	4.7	1.40	0.25	5.22	2.53	1.05	-	9.05				3.62
	20	0.2	35	4.7	1.46	0.33	5.58	2.70	1.06	-	9.67	9.79	51.0	0.95	3.87
Sucrose and raffinose	17 g sucrose + 3 g raffinose	0.2	37	4.7	0.60	0.24	6.38	2.52	1.17	-	10.31	10.3	51.5	1.06	4.12
		0.2	38	4.7	1.07	-	5.57	2.24	1.06	-	8.87				3.55
		0.2	39	4.7	1.29	-	6.41	2.66	1.18	-	10.26				4.10
		0.2	40	4.7	1.09	0.18	6.36	2.62	1.18	-	10.15	11.9	40.5	1.25	4.06
		0.2	41	4.7	0.78	0.34	6.12	2.37	1.17	-	10.00				4.00
		0.2	43	4.7	0.84	0.34	5.52	2.22	1.08	-	9.16				3.66
		0.2	45	4.8	0.74	0.34	5.21	2.03	-	-	7.59	10.8	46.0	0.82	3.04
		0.2	46	4.7	0.78	0.33	5.32	2.07	-	-	7.72				3.09
		0.2	47	4.6	0.54	0.31	5.35	2.05	-	-	7.71				3.08
		0.2	48	4.7	0.83	0.25	5.39	2.05	-	-	7.69				3.08
		0.2	50	4.7	0.41	0.28	5.05	2.08	-	-	7.41				2.96
		0.2	51	4.7	0.83	0.33	5.13	2.13	-	-	7.59	13.2	34.0	1.12	3.04
		0.2	52	4.6	0.45	0.79	5.10	1.97	-	-	7.86				3.14
		0.2	53	4.7	0.48	0.35	5.34	1.97	-	-	7.66	13.1	34.5	1.11	3.06
		0.2	54	4.7	0.19	0.31	5.45	1.99	-	-	7.75				3.10
		0.2	55	4.7	0.22	0.38	5.48	2.04	-	-	7.90				3.16
		0.2	56	4.8	0.32	0.35	5.13	2.00	-	-	7.49				3.00

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