Phaeodactylum tricornutum photorespiration takes part in glycerol metabolism and is important for nitrogen-limited response

Huang, Aiyou; Liu, Lixia; Yang, Chen; Wang, Guangce

doi:10.1186/s13068-015-0256-5

Biotechnology for Biofuels and Bioproducts

Table 1 FLs for amino acids under different labeling experiments

From: Phaeodactylum tricornutum photorespiration takes part in glycerol metabolism and is important for nitrogen-limited response

Fragments	20% U- ¹³ C ^a	30% U- ¹³ C N + ^b	30% U- ¹³ C N- ^c	1,3- ¹³ C N + ^d	1,3- ¹³ C N- ^e
M_ala_057	0.057 ± 0.003	0.180 ± 0.015	0.122 ± 0.006	0.190 ± 0.012	0.161 ± 0.005
M_ala_085	0.076 ± 0.001	0.195 ± 0.013	0.143 ± 0.005	0.202 ± 0.009	0.180 ± 0.004
M_asx_057	0.041 ± 0.001	0.146 ± 0.011	0.094 ± 0.003	0.151 ± 0.016	0.119 ± 0.003
M_asx_085	0.045 ± 0.001	0.149 ± 0.012	0.097 ± 0.003	0.159 ± 0.016	0.125 ± 0.004
M_asx_302	0.042 ± 0.001	0.149 ± 0.010	0.097 ± 0.003	0.148 ± 0.016	0.115 ± 0.004
M_glx_057	0.046 ± 0.001	0.168 ± 0.015	0.140 ± 0.001	0.175 ± 0.013	0.155 ± 0.005
M_glx_085	0.047 ± 0.002	0.170 ± 0.017	0.144 ± 0.004	0.183 ± 0.013	0.165 ± 0.003
M_glx_302	0.068 ± 0.004	0.170 ± 0.014	0.154 ± 0.022	0.157 ± 0.007	0.157 ± 0.015
M_gly_057	0.049 ± 0.002	0.172 ± 0.013	0.294 ± 0.025	0.190 ± 0.008	0.219 ± 0.002
M_gly_085	0.050 ± 0.003	0.173 ± 0.014	0.292 ± 0.025	0.254 ± 0.006	0.362 ± 0.004
M_his_057	-	0.127 ± 0.006	-	0.107 ± 0.029	0.103 ± 0.036
M_his_159	-	0.144 ± 0.026	-	0.127 ± 0.013	0.109 ± 0.008
M_his_302	-	0.134 ± 0.010	-	0.121 ± 0.015	0.096 ± 0.021
M_ile_015	0.041 ± 0.001	0.128 ± 0.006	0.047 ± 0.026	0.124 ± 0.012	0.072 ± 0.043
M_ile_085	0.048 ± 0.002	0.133 ± 0.005	0.069 ± 0.001	0.137 ± 0.014	0.109 ± 0.001
M_leu_015	0.041 ± 0.001	0.135 ± 0.005	0.060 ± 0.013	0.136 ± 0.013	0.109 ± 0.004
M_leu_085	0.047 ± 0.002	0.141 ± 0.005	0.069 ± 0.003	0.150 ± 0.014	0.120 ± 0.002
M_lys_057	0.042 ± 0.002	0.117 ± 0.006	0.073 ± 0.024	0.117 ± 0.012	0.090 ± 0.000
M_lys_159	0.051 ± 0.004	0.119 ± 0.006	0.089 ± 0.046	0.121 ± 0.016	0.095 ± 0.001
M_lys_302	0.064 ± 0.010	0.129 ± 0.005	0.078 ± 0.072	0.092 ± 0.009	0.087 ± 0.002
M_met_057	0.000 ± 0.000	0.148 ± 0.011	0.106 ± 0.031	0.163 ± 0.014	0.161 ± 0.003
M_met_085	0.000 ± 0.000	0.150 ± 0.011	0.142 ± 0.009	0.180 ± 0.017	0.179 ± 0.010
M_phe_057	0.050 ± 0.001	0.142 ± 0.006	0.069 ± 0.006	0.140 ± 0.013	0.118 ± 0.026
M_phe_085	0.048 ± 0.002	0.143 ± 0.006	0.073 ± 0.015	0.139 ± 0.014	0.101 ± 0.013
M_phe_302	0.045 ± 0.003	0.137 ± 0.009	0.056 ± 0.003	0.111 ± 0.012	0.073 ± 0.004
M_phe_sc	0.098 ± 0.002	0.196 ± 0.008	0.366 ± 0.165	0.198 ± 0.014	0.339 ± 0.191
M_pro_057	0.044 ± 0.001	0.140 ± 0.011	0.119 ± 0.018	0.158 ± 0.017	0.121 ± 0.008
M_pro_085	0.042 ± 0.001	0.148 ± 0.012	0.106 ± 0.005	0.162 ± 0.020	0.119 ± 0.001
M_ser_057	0.057 ± 0.001	0.183 ± 0.012	0.313 ± 0.011	0.225 ± 0.009	0.257 ± 0.001
M_ser_085	0.058 ± 0.001	0.184 ± 0.012	0.303 ± 0.014	0.269 ± 0.008	0.346 ± 0.005
M_ser_302	0.057 ± 0.001	0.185 ± 0.012	0.323 ± 0.018	0.199 ± 0.008	0.211 ± 0.001
M_thr_057	0.041 ± 0.001	0.124 ± 0.008	0.070 ± 0.002	0.128 ± 0.017	0.095 ± 0.001
M_thr_085	0.040 ± 0.005	0.125 ± 0.008	0.077 ± 0.006	0.132 ± 0.014	0.100 ± 0.001
M_thr_sc	0.117 ± 0.003	0.180 ± 0.008	0.155 ± 0.030	0.188 ± 0.014	0.167 ± 0.014
M_val_057	0.047 ± 0.002	0.145 ± 0.006	0.073 ± 0.004	0.150 ± 0.013	0.109 ± 0.003
M_val_085	0.054 ± 0.002	0.152 ± 0.005	0.087 ± 0.007	0.152 ± 0.013	0.118 ± 0.002
M_val_302	0.049 ± 0.001	0.152 ± 0.005	0.080 ± 0.005	0.138 ± 0.013	0.101 ± 0.004
M_tyr_057	0.048 ± 0.002	0.140 ± 0.005	0.063 ± 0.005	0.140 ± 0.013	0.103 ± 0.019
M_tyr_085	0.053 ± 0.001	0.143 ± 0.005	0.096 ± 0.041	0.140 ± 0.013	0.150 ± 0.098
M_tyr_302	0.047 ± 0.003	0.140 ± 0.010	0.064 ± 0.005	0.112 ± 0.011	0.078 ± 0.006
average FL	0.047 ± 0.005	0.150 ± 0.008	0.121 ± 0.005	0.157 ± 0.014	0.141 ± 0.007

^aFLs for amino acids under 20% [U-¹³C]glycerol labeling experiments, cultures with NaHCO₃ as carbon source was used as inocula; ^bFLs for amino acids under 30% [U-¹³C]glycerol labeling experiments, nitrogen plus; ^cFLs for amino acids under 30% [U-¹³C]glycerol labeling experiments, nitrogen minus; ^dFLs for amino acids under 50% [1,3-¹³C]glycerol labeling experiments, nitrogen plus; ^eFLs for amino acids under 50% [1,3-¹³C]glycerol labeling experiments, nitrogen minus.

Back to article page

ISSN: 2731-3654

Contact us

General enquiries: journalsubmissions@springernature.com