Table 1 Strains used in this study
From: Anaerobic detoxification of acetic acid in a thermophilic ethanologen
Strain | Description | Source |
|---|---|---|
M0010 | T. saccharolyticum JW/SL-YS485 DSM 8691 | DSMZ |
M0355 | T. saccharolyticum Δpta Δack Δldh | (16) |
M1291 | T. saccharolyticum Δpta Δack Δldh Cth ureABCDEFG | This study |
M1442 | T. saccharolyticum Δpta Δack Δldh Cth ureABCDEFG | This study |
M1667 | T. saccharolyticum Δpta Δack Δldh::Tsa pta ack kanR Cth ureABCDEFG | This study |
M2030 | T. saccharolyticum Δpta Δack Δldh::Tsa pta ack Cac thl Tme thl Tte thl Cac ctfAB Tme ctfAB Cac adc Bam adc kanR Cth ureABCDEFG | This study |
M2202 | T. saccharolyticum adhE*::kanR | This study |
M2203 | T. saccharolyticum adhE::kanR | This study |
M2204 | T. saccharolyticum hfs*::kanR | This study |
M2205 | T. saccharolyticum hfs::kanR | This study |
M2212 | T. saccharolyticum Δpta Δack Δldh::Tsa pta ack Tte thl Tme ctfAB Bam adc kanR Cth ureABCDEFG | This study |
Top10 | E. coli cloning strain | Invitrogen, Madison, WI |
FY2 | S. cerevisiae cloning strain | (37) |
| Â | Re-sequenced strains with improved growth rate | Â |
M0694 | T. saccharolyticum Δpta Δack Δldh continuous cultured adapted | This study |
M0699 | T. saccharolyticum Δpta Δack Δldh continuous cultured adapted | This study |
M0700 | T. saccharolyticum Δpta Δack Δldh continuous cultured adapted | This study |
M0731 | T. saccharolyticum Δpta Δack Δldh mutation and serial transfer | This study |
M0734 | T. saccharolyticum Δpta Δack Δldh mutation and serial transfer | This study |
M0863 | T. saccharolyticum Δpta Δack Δldh mutation and serial transfer | This study |