|  |
Units in mM
| Â |
---|
Strain
|
Genotype
|
Cellobiose consumed
|
Lactate
|
Acetate
|
Ethanol
|
Carbon recovery (%)
|
---|
M0010 | WT | 28.2 ± 0.6 | 13.8 ± 0.5 | 34.7 ± 0.7 | 63.5 ± 2.3 | 99 |
M2202 |
adhE*::kan
R
| 30.2 ± 0.0 | 2.4 ± 0.1 | 42.3 ± 0.3 | 76.5 ± 0.6 | 100 |
M2203 |
adhE::kan
R
| 27.7 ± 2.6 | 1.4 ± 0.2 | 29.0 ± 1.6 | 78.4 ± 8.6 | 98 |
M2204 |
hfs*::kan
R
| 29.9 ± 0.1 | 20.3 ± 0.5 | 4.2 ± 0.1 | 91.7 ± 0.6 | 97 |
M2205 |
hfs::kan
R
| 28.1 ± 0.7 | 9.9 ± 2.2 | 29.8 ± 1.1 | 67.9 ± 2.7 | 96 |
- *mutations found in evolved strain M0863 introduced by homologous recombination. Data are average of four replicates, anaerobic TSC7 medium, 55°C, 24-h fermentation of 30.4 ± 0.7 mM cellobiose. Mutations were created via homologous recombination of a kanamycin resistance marker downstream of the hfs operon that contained mutations in the upstream flanking region. M2202 - kanR inserted 3′ of the adhE gene, with the M0863 mutant adhE sequence. M2203 - kanR inserted 3′ of the adhE gene, with the WT adhE sequence. M2204 - kanR inserted 3′ of the hfs gene operon, with the M0863 mutant hfs sequence. M2205 - kanR inserted 3′ of the hfs gene operon, with the WT hfs sequence.