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Table 3 Re-introduction of spontaneous adhE and hfs hydrogenase mutations that occurred during growth adaptation of the Δldh , Δpta Δack ethanologen strain M0863 into wildtype (WT) T. saccharolyticum

From: Anaerobic detoxification of acetic acid in a thermophilic ethanologen

  

Units in mM

 

Strain

Genotype

Cellobiose consumed

Lactate

Acetate

Ethanol

Carbon recovery (%)

M0010

WT

28.2 ± 0.6

13.8 ± 0.5

34.7 ± 0.7

63.5 ± 2.3

99

M2202

adhE*::kan R

30.2 ± 0.0

2.4 ± 0.1

42.3 ± 0.3

76.5 ± 0.6

100

M2203

adhE::kan R

27.7 ± 2.6

1.4 ± 0.2

29.0 ± 1.6

78.4 ± 8.6

98

M2204

hfs*::kan R

29.9 ± 0.1

20.3 ± 0.5

4.2 ± 0.1

91.7 ± 0.6

97

M2205

hfs::kan R

28.1 ± 0.7

9.9 ± 2.2

29.8 ± 1.1

67.9 ± 2.7

96

  1. *mutations found in evolved strain M0863 introduced by homologous recombination. Data are average of four replicates, anaerobic TSC7 medium, 55°C, 24-h fermentation of 30.4 ± 0.7 mM cellobiose. Mutations were created via homologous recombination of a kanamycin resistance marker downstream of the hfs operon that contained mutations in the upstream flanking region. M2202 - kanR inserted 3′ of the adhE gene, with the M0863 mutant adhE sequence. M2203 - kanR inserted 3′ of the adhE gene, with the WT adhE sequence. M2204 - kanR inserted 3′ of the hfs gene operon, with the M0863 mutant hfs sequence. M2205 - kanR inserted 3′ of the hfs gene operon, with the WT hfs sequence.