Contribution of a family 1 carbohydrate-binding module in thermostable glycoside hydrolase 10 xylanase from Talaromyces cellulolyticus toward synergistic enzymatic hydrolysis of lignocellulose

Table 1 Substrate specificities of purified intact and mutant Xyl10As toward different substrates

Substrates	Xyl10Awt (A) (U/nmol)	Xyl10AdC (B) (U/nmol)	Xyl10AdLC (C) (U/nmol)	(B)/(A)	(C)/(A)
Birchwood xylan	10.6	15.1	13.9	1.42	1.31
Wheat arabinoxylan	21.9	35.5	28.2	1.62	1.29
Insoluble oat-spelt xylan	2.53	3.85	3.66	1.52	1.45
PNP-lactose	0.048	0.064	0.061	1.34	1.28
PNP-cellobiose	0.251	0.393	0.342	1.56	1.36
PNP-xylose	0.022	0.028	0.026	1.25	1.18
WDM-RS^a	0.094	0.054	0.066	0.57	0.70

Activities were determined at 45°C (pH 5.0). ^aActivities for WDM-RS were determined at 50°C (pH5.0). PNP-lactose, p-nitrophenyl-β-D-lactoside; PNP-cellobiose, p-nitrophenyl-β-D-cellobioside; PNP-xylose, p-nitrophenyl-β-D-xyloside; WDM-RS, wet disc-mill treated rice straw.

ISSN: 2731-3654