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Table 1 Substrate specificities of purified intact and mutant Xyl10As toward different substrates

From: Contribution of a family 1 carbohydrate-binding module in thermostable glycoside hydrolase 10 xylanase from Talaromyces cellulolyticus toward synergistic enzymatic hydrolysis of lignocellulose

Substrates Xyl10Awt (A) (U/nmol) Xyl10AdC (B) (U/nmol) Xyl10AdLC (C) (U/nmol) (B)/(A) (C)/(A)
Birchwood xylan 10.6 15.1 13.9 1.42 1.31
Wheat arabinoxylan 21.9 35.5 28.2 1.62 1.29
Insoluble oat-spelt xylan 2.53 3.85 3.66 1.52 1.45
PNP-lactose 0.048 0.064 0.061 1.34 1.28
PNP-cellobiose 0.251 0.393 0.342 1.56 1.36
PNP-xylose 0.022 0.028 0.026 1.25 1.18
WDM-RSa 0.094 0.054 0.066 0.57 0.70
  1. Activities were determined at 45°C (pH 5.0). aActivities for WDM-RS were determined at 50°C (pH5.0). PNP-lactose, p-nitrophenyl-β-D-lactoside; PNP-cellobiose, p-nitrophenyl-β-D-cellobioside; PNP-xylose, p-nitrophenyl-β-D-xyloside; WDM-RS, wet disc-mill treated rice straw.