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Table 2 Kinetic parameters for hydrolysis of birchwood xylan by intact and mutant Xyl10As

From: Contribution of a family 1 carbohydrate-binding module in thermostable glycoside hydrolase 10 xylanase from Talaromyces cellulolyticus toward synergistic enzymatic hydrolysis of lignocellulose

Enzyme K m (mg mL −1 ) k cat (s −1 ) k cat / K m (s −1 mg −1 mL −1 )
Xyl10Awt 2.51 ± 0.42 232 ± 12.4 92.2 ± 16.2
Xyl10AdC 2.80 ± 0.18 359 ± 7.8 128 ± 8.9
Xyl10AdLC 2.59 ± 0.14 310 ± 5.4 120 ± 6.7
  1. Reactions were performed at 45°C (pH 5.0).