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Table 8 Comparison and validation of the proteomic data with earlier reported proteomic work

From: Complex and extensive post-transcriptional regulation revealed by integrative proteomic and transcriptomic analysis of metabolite stress response in Clostridium acetobutylicum

Work

Brief description

Proteins identified

Proteins found in this work (iTRAQ)

Key findings

Comparison with mRNA expression

Schaffer et al. (2002)

Solventogenesis

130

All 130 proteins have been identified under butanol/butyrate stress

Proteins involved in the solventogenic pathway

Northern analysis (selected few)

Sullivan and Bennett (2006)

Acidogenesis and solventogenesis

23

22 proteins were observed in this study

Proteins expressed during the onset of solventogenesis

With microarray data from Tomas and Alsaker (2004)

Mao et al. (2010)

Cytoplasmic proteins (DSM 1731 and Rh8 tolerant mutant)

564

86 out of the 102 differentially expressed proteins were identified

Proteins that play a role in solvent toxicity tolerance

qRT-PCR of selected differentially expressed protein

Janssen et al. (2010)

Continuous culture of WT in acidogenic and solventogenic phases

178 + 205

178 acidogenic proteins and 205 solventogenic proteins

Proteins that are expressed under different metabolic phases

DNA microarrays

Mao et al. (2011)

Membrane protein DSM 1731 and RH8 tolerant phenotype

341

23 out of the 33 differentially expressed membrane proteins were identified

Membrane proteins that were differentially expressed in the butanol hyper-tolerant mutant

None

Sivagnanam et al. (2011)

CAC proteome under glucose and xylose utilization

717 (glucose) 826 (xylose)

22 of the 23 differentially expressed proteins were identified

Proteins that correspond to differential utilization of carbon sources

None

Bai and Ji (2012)

Phophoproteome of C. acetobutylicum

61 phospho-proteins

57/61 phosphor proteins were also identified in our work

Proteins with post translational modifications (PTMs) and their role in stress response

None

Sivagnanam et al. (2012)

Protein interaction network using STRING and CYTOSCAPE

217 proteins were used to construct a PPI network with 1947 interactions

N/A

Construction of PPI network to identify regulatory interactions

None

Jang et al. (2014)

Acidogenesis and solventogenesis. WT, M5, and M5 + pIMP

56

All were also identified by us

Proteins differentially expressed during acidogenic and solventogenic phases

None