Enzyme activity highlights the importance of the oxidative pentose phosphate pathway in lipid accumulation and growth of Phaeodactylum tricornutum under CO2 concentration

Table 3 List of primers used in the real-time PCR analysis

Primers	Sequence (5′–3′)	Annealing temperature (°C)	Amplicon size (bp)
RPS (ribosomal protein small subunit 30S)	Sense: CGAAGTCAACCAGGAAACCAA	56	166
RPS (ribosomal protein small subunit 30S)	Antisense: GTGCAAGAGACCGGACATACC	56	166
TBP (TATA box binding protein)	Sense: ACCGGAGTCAAGAGCACACAC	56	175
TBP (TATA box binding protein)	Antisense: CGGAATGCGCGTATACCAGT	56	175
Rubisco (rbc S)	Sense: ACTCTGCTGGTGCTGTGCG	56	214
Rubisco (rbc S)	Antisense: TGGGATTGGCGTCGTTCTT	56	214
PRK	Sense: GAAGTTTGCTGTCTTTGCCTCT	56	139
PRK	Antisense: GATGGGTGTCTGTCCCTCCT	56	139
PGK	Sense: TGGTGGTGGCGACTCTGT	56	156
PGK	Antisense: TACGCATTCCCGGCTTAC	56	156
G6PDH	Sense: GCGAGAAATGGCACAAGG	56	180
G6PDH	Antisense: GTTCATCGCAGTCGGGAGA	56	180
6PGDH	Sense: GTTCACCGTTGCCGTTTG	56	143
6PGDH	Antisense: CGACTTTCCGAGGCTTGCTG	56	143

ISSN: 2731-3654