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Table 2 Comparison of the CO2-fixation rates of autotrophic and heterotrophic CO2-fixing microbes

From: Quantitative analysis of an engineered CO2-fixing Escherichia coli reveals great potential of heterotrophic CO2 fixation

  Species CO2-fixation rate (mg L−1 h−1) Biomass concentration (g DCW L−1) Specific CO2-fixation ratea (mg g DCW−1 h−1) CO2 concentration (%) Culture condition References
Autotrophic microbes  
Microalgae Chlorella pyrenoidosa SJTU-2 10.8 1.5 7.3 10 1 L flask with 800 mL WV [52]
  Dunaliella tertiolecta SAD-13.86 11.0 2.1 5.2 10 11 Lfermentor with 8 L WV [53]
  Botryococcus braunii SAG-30.81 21.0 3.1 6.8 10 11 L fermentor with 8 L WV [53]
  Scenedesmus obliquus SJTU-3 12.1 1.8 6.6 10 1 L flask with 800 mL WV [52]
  Scenedesmus sp. NIER-10060 25.5 2.7 9.4 15 Photobioreactor [54]
  Chlorella vulgaris LEB-104 10.5 1.9 5.4 10 11 L fermentor with 8 L WV [53]
  Chlorella Vulgaris NIER-10003 19.2 1.9 10.2 15 Photobioreactor [54]
  Chlorella vulgaris 53.0 5.7 9.3 5 Photobioreactorc [55]
Cyanobacteria Spirulina sp. 17.0a 4.8 3.5 6 2 L vertical tubular photobioreactor with 1.8 L WV [56]
  Microcystis aeruginosa NIER-10037 20.4 2.3 8.8 15 Photobioreactor [54]
  Microcystis ichthyoblabe NIER-10040 21.7 2.2 9.8 15 Photobioreactor [54]
  Anabaena sp. ATCC 33047 60.4 2.7 22.4 0.03b Glass bubble column photobioreactor [57]
  Aphanothece microscopica 109.0 5.1 21.4 15 Glass bubble column photobioreactor [58]
Non-green algae Phaeodactylum tricornutum 147.0 6.2 23.7 40 Photobioreactor [59]
Heterotrophic microbes
Bacteria E. coli JB 5.8 6.1c 0.95 0.03 3 L fermentor with 1 L WV [19]
  E. coli BL21(DE3)/PET-RBC-PRK 13.3 0.82 16.2 5 1 L flask with 200 mL WV This study
  E. coli BL21(DE3)/PET-RBC-PRK-CA 19.6 0.87 22.5 5 1 L flask with 200 mL WV This study
  1. DCW dry cell weight, WV working volume
  2. aCalculated by the CO2-fixation rate in the unit of mg L−1 h−1 divided by the biomass concentration in the unit of g DCW L−1
  3. bCalculated by multiplying the reported OD600 (17.63) by our experimentally determined dry cell weight of E. coli (0.35 g L−1 OD600 −1)
  4. cSequential photobioreactor using recycle water