Table 1 Strains and plasmids used in this study
Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
C. glutamicum strains | ||
WT | Wild type (ATCC13032) | [76] |
Δcg1497 | In-frame deletion of cg1497 in C. glutamicum WT | This work |
ΔhdpA | In-frame deletion of hdpA (cg2474) in C. glutamicum WT | This work |
Δcg1497ΔhdpA | In-frame deletion of hdpA (cg2474) in C. glutamicum Δcg1497 | This work |
ΔhdpAΔldh | In-frame deletion of ldh (cg3219) in C. glutamicum ΔhdpA | This work |
Plasmids | ||
pK19mobsacB | Kana, mobilizable E. coli vector for the construction of insertion and deletion mutants of C. glutamicum (oriV, sacB, lacZ) | [75] |
pEKEx3 | Speca; C. glutamicum/E. coli shuttle vector (Ptac, lacI b; pBL1, OriVC.g., OriVE.c.) | [45] |
pVWEx1 | Kana; C. glutamicum/E. coli shuttle vector for regulated gene expression (Ptac, lacIb, pHM1519, oriVC.g., oriVE.c.) | [77] |
pK19mobsacB-Δcg1497 | Kana, pK19mobsacB with the deletion construct for cg1497 | This work |
pK19mobsacB-ΔhdpA | Kana, pK19mobsacB with the deletion construct for hdpA (cg2474) | This work |
pK19mobsacB-Δldh | Kana, pK19mobsacB with the deletion construct for ldh (cg3219) | [28] |
pEKEx3-mgsA-gldA | Derived from pEKEx3 for IPTG-inducible overexpression of mgsA and gldA from E. coli with artificial ribosome binding site in front of each gene | This work |
pEKEx3-mgsA-yqhD-gldA | Derived from pEKEx3 for IPTG-inducible overexpression of mgsA, yqhD, and gldA from E. coli with artificial ribosome binding site in front of each gene | This work |
pEKEx3-mgsA-yqhD-fucO-gldA | Derived from pEKEx3 for IPTG-inducible overexpression of mgsA, yqhD, fucO, and gldA from E. coli with artificial ribosome binding site in front of each gene | This work |
pVWEx1-ppdABC | Derived from pEKEx3 for IPTG-inducible overexpression of ppdABC from K. oxytoca DSM4798 with artificial ribosome binding site in front of the gene cluster | This work |