Skip to main content

Table 2 Plasmids used in this study

From: Metabolic engineering of microbes for branched-chain biodiesel production with low-temperature property

Plasmids

Replication origin

Overexpressed genes

Resistance

Reference

pDG16

pBR322

PT7: alsS

Kan

This study

pDG17

pBR322

PT7: ilvC

Kan

This study

pDG18

pBR322

PT7: ilvD

Kan

This study

pDG19

pBR322

PT7: ilvC and ilvD

Kan

This study

pDG20

pBR322

PT7: alsS, ilvC, and ilvD

Kan

This study

pDG102

pBR322

PT7: aro10, adh2, ws/dgat, and fadD

Kan

Reference [31]

pDG104

pBR322

PT7: ws/dgat, aro10, adh2, fadD, alsS, ilvC, and ilvD

Kan

This study

pTL30

pBR322

PT7: tesA’

Kan

Reference [14]

pXC002

pSC101

PT7

Amp

Not published

pDG105

pSC101

PT7: tesA’

Amp

This study

pDG25

pBBR1

PT7: bckd

Cam

This study

pDG110

pBBR1

PT7: fabHB and bckd

Cam

This study

pDG10

pBR322

PGAP: his4

G418 and His

This study

pDG11

pBR322

PGAP: ws/dgat

G418

This study

pDG12

pBR322

PGAP: aro10

G418

This study

pDG13

pBR322

PGAP: adh2

G418

This study

pDG14

pBR322

PGAP: ws/dgat

G418 and His

This study

pDG15

pBR322

PGAP: ws/dgat and aro10

G418 and His

This study

pDG103

pBR322

PGAP: ws/dgat, aro10, and adh2

G418 and His

This study