Fig. 1

Oxidative cleavage of cellulose by P. anserina AA9 LPMOs. a Schematic representation of the modularity of P. anserina AA9 LPMOs based on CAZy annotation. b Quantification by HPAEC analysis of the soluble sugars released by 4.4 μM PaLPMO and 1.2 U.ml⁻¹ of PaCDHB, at 50 °C for 16 h. The concentration of each sugar was determined by integration of the peak area and comparison with a standard curve. Values are the mean of three biological replicates (n = 3). Error bars correspond to one cumulated SD (error bar = ±SDtot; with SDtot = √(SD₁ ² + SD₂ ² + …). c HPAEC chromatograms showing products generated from cellulose with PaLPMO9A, PaLPMO9H, and PaLPMO9E. The peak annotations are based on comparison with oligosaccharides standards oxidized at the C1 position (DP2ox-DP5 ox)