Fig. 2

Analysis of degradation products generated by PaLPMO9A and PaLPMO9H. The HPAEC chromatograms of the oligosaccharides released upon degradation of 0.1 % PASC with 4.4 μM PaLPMO in the presence of 1 mM ascorbate, at 50 °C for 16 h (in black) followed by the incubation with 1.2 U.ml⁻¹ of PaCDHB at 50 °C for 8 h (in red). The peak annotations are based on comparison with oligosaccharides standards oxidized at the C1 position (DP2ox-DP5ox). Coelution of DP1ox with DP3 and DP2ox with DP6 was observed. Peaks eluting at 27, 38, and 41 min are annotated with dotted lines