Substrate specificity and regioselectivity of fungal AA9 lytic polysaccharide monooxygenases secreted by Podospora anserina

Table 1 Biochemical and enzymatic characteristics of the enzymes used in this study

Enzymes	GenBank ID	Modularity	Predicted function	SA^a (U.g⁻¹)	Competitive inhibition assay (% of residual activity)
Enzymes	GenBank ID	Modularity	Predicted function	SA^a (U.g⁻¹)	PASC	CMC
PaCDHB	CAP61651	AA3_1-AA8	CDH	8.6	N.D.	N.D.
PaLPMO9A	CAP73254	AA9-CBM1	LPMO	0.010 ± 0.001	7.6 ± 1.1	97.9 ± 2.1
PaLPMO9D	CAP66744	AA9	LPMO	0.083 ± 0.005	75.1 ± 7.5	73.2 ± 8.5
PaLPMO9E	CAP67740	AA9-CBM1	LPMO	1.95 ± 0.04	18.4 ± 4.2	47.0 ± 1.3
PaLPMO9F	CAP71839	AA9	LPMO	0.028 ± 0.003	16.9 ± 1.9	100.0 ± 4.8
PaLPMO9G	CAP73072	AA9	LPMO	n.d.	N.D.	N.D.
PaLPMO9H	CAP61476	AA9-CBM1	LPMO	0.42 ± 0.03	7.2 ± 1.4	6.1 ± 0.8

n.d. no production of H₂O₂ detected using the Amplex Red assay, N.D. not determined
^aSpecific activity was measured using DCPIP (pH 5, 30 °C) for PaCDHB and the Amplex Red assay for PaLPMOs (pH 6, 30 °C)

ISSN: 2731-3654