Fig. 6
Comparison of glucose and xylose recovery after enzymatic saccharification as a percent of original biomass for [C2C1im][OAc] pretreatment. Glucose and xylose recovery after 70 °C for 5 h and 140 °C for 3 h compared to the untreated (ut) for all of the Arabidopsis lines. There was an significant difference in total sugar released per starting biomass between the Arabidopsis lines at each pretreatment temperature, untreated (F(3,12) = 72.44, P < 0.0001), 70 °C (F(3,12) = 19.45, P < 0.0005), and 140 °C (F(3,12) = 5.86, P < 0.05). This was in part due to significant differences between groups in glucose recovery per starting biomass for all three pretreatment conditions untreated (F(3,12) = 47.2, P < 0.0001), 70 °C (F(3,12) = 7.86, P < 0.01), and 140 °C (F(3,12) = 6.62, P < 0.01). There was also significant difference in xylose recovery per starting biomass between the lines for two of the three pretreatment conditions untreated (F(3,12) = 134.12, P < 0.0001) and 70 °C (F(3,12) = 404.71, P < 0.0001). There was not a significant difference in xylose release per starting biomass at 140 °C (F(3,12) = 3.43, P = 0.07). ANOVA with a Tukey’s HSD post-hoc test and the Tukey’s HSD post-hoc test are shown in the figure for the comparison to WT (total sugar, P < 0.05, *; P < 0.01, **; glucose, P < 0.05, +; P < 0.01, ++; xylose, P < 0.05, −; P < 0.01, --), additional post-hoc test comparisons reported in Additional file 9: Table S1 and S2