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Table 2 Percent recovered solid composition after pretreatment at 140 °C for 3 h with [C2C1im][OAc] at 10 % (w/w) biomass loading as a percent of starting biomass

From: Restricting lignin and enhancing sugar deposition in secondary cell walls enhances monomeric sugar release after low temperature ionic liquid pretreatment

  Solids recovery 140 °C 3 h  
  % Glucose % Xylose % Lignin % ASR
WT 101 ± 6 47 ± 3 82 ± 5 17 ± 2
LLL 86 ± 7 39 ± 3 68 ± 6 23 ± 1*
LLHPL1 70 ± 3** 34 ± 1* 81 ± 14 38 ± 5**
LLHPL2 74 ± 10** 44 ± 6 70 ± 13 28 ± 3**
Pretreated solids composition 140 °C for 3 h
  % Total solids % Glucose % Xylose % Lignin
WT 52 ± 3 51 ± 1 10 ± 2 30 ± 2
LLL 43 ± 4 46 ± 2 10 ± 2 20 ± 2*
LLHPL1 52 ± 2 41 ± 6 11 ± 1 21 ± 4**
LLHPL2 44 ± 6 37 ± 3 12 ± 3 21 ± 3**
  1. All values presented as ±SD. There was an overall significant difference in % recovery of glucose, xylose, and ASR (acid soluble residue, ash, and protein) in the recovered solids, F(3,12) = 12.86, P < 0.002, F(3,12) = 7.37, P < 0.01 and F(3,12) = 32.87, P < 0.0001. There was a non-significant difference in the lignin recovery in the solids, F(3,12) = 1.03, P = 0.43. Composition of recovered solids after pretreatment with [C2C1im][OAc] for 140 °C 3 h at 10 % (w/w) biomass loading. Glucose, xylose, and lignin reported as a percent of recovered biomass ±SD. There was an overall significant difference in % total solids and lignin, F(3,12) = 5.08, P < 0.05, F(3,12) = 9.74, P < 0.005. There was no overall significance for the % composition glucose or xylose F(3,12) = 6.22, P = 0.05, F(3,12) = 0.35, P = 0.79. ANOVA with a Tukey’s HSD post-hoc test was used to determine overall statistics, and results of the comparison to WT from the post-hoc test are shown in the table
  2. * P < 0.05; ** P < 0.01