|  | Solids recovery | 140 °C 3 h |  |
|---|
| Â | % Glucose | % Xylose | % Lignin | % ASR |
|---|
WT
| 101 ± 6 | 47 ± 3 | 82 ± 5 | 17 ± 2 |
LLL
| 86 ± 7 | 39 ± 3 | 68 ± 6 | 23 ± 1*
|
LLHPL1
| 70 ± 3**
| 34 ± 1*
| 81 ± 14 | 38 ± 5**
|
LLHPL2
| 74 ± 10**
| 44 ± 6 | 70 ± 13 | 28 ± 3**
|
Pretreated solids composition 140 °C for 3 h |
| Â | % Total solids | % Glucose | % Xylose | % Lignin |
WT
| 52 ± 3 | 51 ± 1 | 10 ± 2 | 30 ± 2 |
LLL
| 43 ± 4 | 46 ± 2 | 10 ± 2 | 20 ± 2*
|
LLHPL1
| 52 ± 2 | 41 ± 6 | 11 ± 1 | 21 ± 4**
|
LLHPL2
| 44 ± 6 | 37 ± 3 | 12 ± 3 | 21 ± 3**
|
- All values presented as ±SD. There was an overall significant difference in % recovery of glucose, xylose, and ASR (acid soluble residue, ash, and protein) in the recovered solids, F(3,12) = 12.86, P < 0.002, F(3,12) = 7.37, P < 0.01 and F(3,12) = 32.87, P < 0.0001. There was a non-significant difference in the lignin recovery in the solids, F(3,12) = 1.03, P = 0.43. Composition of recovered solids after pretreatment with [C2C1im][OAc] for 140 °C 3 h at 10 % (w/w) biomass loading. Glucose, xylose, and lignin reported as a percent of recovered biomass ±SD. There was an overall significant difference in % total solids and lignin, F(3,12) = 5.08, P < 0.05, F(3,12) = 9.74, P < 0.005. There was no overall significance for the % composition glucose or xylose F(3,12) = 6.22, P = 0.05, F(3,12) = 0.35, P = 0.79. ANOVA with a Tukey’s HSD post-hoc test was used to determine overall statistics, and results of the comparison to WT from the post-hoc test are shown in the table
-
*
P < 0.05; **
P < 0.01
