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Table 3 Enzymatic saccharification efficiency of Arabidopsis engineered line versus pretreatment condition

From: Restricting lignin and enhancing sugar deposition in secondary cell walls enhances monomeric sugar release after low temperature ionic liquid pretreatment

Enzymatic saccharification 10 % loading for 72 h
   % Glucose % Xylose % Glucose recovery % Xylose recovery
WT Untreated 31 ± 3 17 ± 3 31 ± 3 17 ± 3
  10 %, 70 °C, 5 h 67 ± 20 1.0 ± 0.3 62 ± 11 1.0 ± 0.2
  10 %, 140 °C, 3 h 84 ± 6 87 ± 2 84 ± 1 41 ± 2
LLL Untreated 46 ± 4** 33 ± 1** 46 ± 4** 33 ± 1**
  10 %, 70 °C, 5 h 76 ± 7 46 ± 5** 76 ± 5 46 ± 4**
  10 %, 140 °C, 3 h 95 ± 4 92 ± 7 82 ± 4 35 ± 4**
LLHPL1 Untreated 48.4 ± 0.7** 30.2 ± 0.2** 48 ± 0.4** 30 ± 0.3**
  10 %, 70 °C, 5 h 79 ± 4** 58 ± 3** 63 ± 4 48 ± 2**
  10 %, 140 °C, 3 h 99 ± 7* 83 ± 9 69 ± 3* 30 ± 3
LLHPL2 Untreated 53 ± 3** 31 ± 1** 53 ± 3** 31 ± 1**
  10 %, 70 °C, 5 h 81 ± 4** 55 ± 5** 79 ± 1* 58 ± 1**
  10 %, 140 °C, 3 h 117 ± 6** 89 ± 6 87 ± 8 39 ± 2
  1. Enzymatic saccharification efficiency reported as percent of theoretical in the saccharification (released as percent from pretreated biomass) and final recovery % from concentration in initial solids (sugar recovery * enzymatic efficiency), from the cellulose and hemicellulose mixtures CTec2 and HTec2 (20 mg/g and 2 mg/g loading for 72 h). All values presented as ±SD. There was an overall significant difference of the % glucose and xylose released from untreated biomass during enzymatic saccharification between the WT and the three engineered lines, F(3,12) = 30.59, P < 0.0001, F(3,12) = 66.83, P < 0.0001, xylose for the 70 °C pretreated biomass, F(3,12) = 139.36, P < 0.0001, and glucose for the 140 °C pretreated biomass, F(3,12) = 18.57, P < 0.001. There was a non-significant differences for the % saccharification efficiency for glucose for the 70 °C pretreatment F(3,12) = 0.95, P = 0.46 and for xylose for the 140 °C pretreatment F(3,12) = 0.85, P = 0.50. There were significant differences both the glucose and xylose recoveries at each pretreatment condition, untreated (F(3,12) = 30.6, P < 0.0001, F(3,12) = 66.8, P < 0.0001), 70 °C (F(3,12) = 4.35, P < 0.05 F(3,12) = 355.29, P < 0.0001) and 140 °C (F(3,12) = 7.93, P < 0.01, F(3,12) = 9.38, P < 0.01). ANOVA with a Tukey’s HSD post-hoc test was used to determine overall statistics, and results of the comparison to WT from the post-hoc test are shown in the table
  2. * P < 0.05; ** P < 0.01