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Table 3 Enzymatic saccharification efficiency of Arabidopsis engineered line versus pretreatment condition

From: Restricting lignin and enhancing sugar deposition in secondary cell walls enhances monomeric sugar release after low temperature ionic liquid pretreatment

Enzymatic saccharification 10 % loading for 72 h

  

% Glucose

% Xylose

% Glucose recovery

% Xylose recovery

WT

Untreated

31 ± 3

17 ± 3

31 ± 3

17 ± 3

 

10 %, 70 °C, 5 h

67 ± 20

1.0 ± 0.3

62 ± 11

1.0 ± 0.2

 

10 %, 140 °C, 3 h

84 ± 6

87 ± 2

84 ± 1

41 ± 2

LLL

Untreated

46 ± 4**

33 ± 1**

46 ± 4**

33 ± 1**

 

10 %, 70 °C, 5 h

76 ± 7

46 ± 5**

76 ± 5

46 ± 4**

 

10 %, 140 °C, 3 h

95 ± 4

92 ± 7

82 ± 4

35 ± 4**

LLHPL1

Untreated

48.4 ± 0.7**

30.2 ± 0.2**

48 ± 0.4**

30 ± 0.3**

 

10 %, 70 °C, 5 h

79 ± 4**

58 ± 3**

63 ± 4

48 ± 2**

 

10 %, 140 °C, 3 h

99 ± 7*

83 ± 9

69 ± 3*

30 ± 3

LLHPL2

Untreated

53 ± 3**

31 ± 1**

53 ± 3**

31 ± 1**

 

10 %, 70 °C, 5 h

81 ± 4**

55 ± 5**

79 ± 1*

58 ± 1**

 

10 %, 140 °C, 3 h

117 ± 6**

89 ± 6

87 ± 8

39 ± 2

  1. Enzymatic saccharification efficiency reported as percent of theoretical in the saccharification (released as percent from pretreated biomass) and final recovery % from concentration in initial solids (sugar recovery * enzymatic efficiency), from the cellulose and hemicellulose mixtures CTec2 and HTec2 (20 mg/g and 2 mg/g loading for 72 h). All values presented as ±SD. There was an overall significant difference of the % glucose and xylose released from untreated biomass during enzymatic saccharification between the WT and the three engineered lines, F(3,12) = 30.59, P < 0.0001, F(3,12) = 66.83, P < 0.0001, xylose for the 70 °C pretreated biomass, F(3,12) = 139.36, P < 0.0001, and glucose for the 140 °C pretreated biomass, F(3,12) = 18.57, P < 0.001. There was a non-significant differences for the % saccharification efficiency for glucose for the 70 °C pretreatment F(3,12) = 0.95, P = 0.46 and for xylose for the 140 °C pretreatment F(3,12) = 0.85, P = 0.50. There were significant differences both the glucose and xylose recoveries at each pretreatment condition, untreated (F(3,12) = 30.6, P < 0.0001, F(3,12) = 66.8, P < 0.0001), 70 °C (F(3,12) = 4.35, P < 0.05 F(3,12) = 355.29, P < 0.0001) and 140 °C (F(3,12) = 7.93, P < 0.01, F(3,12) = 9.38, P < 0.01). ANOVA with a Tukey’s HSD post-hoc test was used to determine overall statistics, and results of the comparison to WT from the post-hoc test are shown in the table
  2. * P < 0.05; ** P < 0.01