Enzymatic saccharification 10 % loading for 72 h |
---|
 |  | % Glucose | % Xylose | % Glucose recovery | % Xylose recovery |
---|
WT
| Untreated | 31 ± 3 | 17 ± 3 | 31 ± 3 | 17 ± 3 |
 | 10 %, 70 °C, 5 h | 67 ± 20 | 1.0 ± 0.3 | 62 ± 11 | 1.0 ± 0.2 |
 | 10 %, 140 °C, 3 h | 84 ± 6 | 87 ± 2 | 84 ± 1 | 41 ± 2 |
LLL
| Untreated | 46 ± 4**
| 33 ± 1**
| 46 ± 4**
| 33 ± 1**
|
 | 10 %, 70 °C, 5 h | 76 ± 7 | 46 ± 5**
| 76 ± 5 | 46 ± 4**
|
 | 10 %, 140 °C, 3 h | 95 ± 4 | 92 ± 7 | 82 ± 4 | 35 ± 4**
|
LLHPL1
| Untreated | 48.4 ± 0.7**
| 30.2 ± 0.2**
| 48 ± 0.4**
| 30 ± 0.3**
|
 | 10 %, 70 °C, 5 h | 79 ± 4**
| 58 ± 3**
| 63 ± 4 | 48 ± 2**
|
 | 10 %, 140 °C, 3 h | 99 ± 7*
| 83 ± 9 | 69 ± 3*
| 30 ± 3 |
LLHPL2
| Untreated | 53 ± 3**
| 31 ± 1**
| 53 ± 3**
| 31 ± 1**
|
 | 10 %, 70 °C, 5 h | 81 ± 4**
| 55 ± 5**
| 79 ± 1*
| 58 ± 1**
|
 | 10 %, 140 °C, 3 h | 117 ± 6**
| 89 ± 6 | 87 ± 8 | 39 ± 2 |
- Enzymatic saccharification efficiency reported as percent of theoretical in the saccharification (released as percent from pretreated biomass) and final recovery % from concentration in initial solids (sugar recovery * enzymatic efficiency), from the cellulose and hemicellulose mixtures CTec2 and HTec2 (20 mg/g and 2 mg/g loading for 72 h). All values presented as ±SD. There was an overall significant difference of the % glucose and xylose released from untreated biomass during enzymatic saccharification between the WT and the three engineered lines, F(3,12) = 30.59, P < 0.0001, F(3,12) = 66.83, P < 0.0001, xylose for the 70 °C pretreated biomass, F(3,12) = 139.36, P < 0.0001, and glucose for the 140 °C pretreated biomass, F(3,12) = 18.57, P < 0.001. There was a non-significant differences for the % saccharification efficiency for glucose for the 70 °C pretreatment F(3,12) = 0.95, P = 0.46 and for xylose for the 140 °C pretreatment F(3,12) = 0.85, P = 0.50. There were significant differences both the glucose and xylose recoveries at each pretreatment condition, untreated (F(3,12) = 30.6, P < 0.0001, F(3,12) = 66.8, P < 0.0001), 70 °C (F(3,12) = 4.35, P < 0.05 F(3,12) = 355.29, P < 0.0001) and 140 °C (F(3,12) = 7.93, P < 0.01, F(3,12) = 9.38, P < 0.01). ANOVA with a Tukey’s HSD post-hoc test was used to determine overall statistics, and results of the comparison to WT from the post-hoc test are shown in the table
-
*
P < 0.05; **
P < 0.01