Skip to main content

Advertisement

Table 5 Rate of enzymatic saccharification as calculated by release during the first 30 min of enzymatic hydrolysis with both the cellulase and hemicellulase mixtures CTec2 and HTec2

From: Restricting lignin and enhancing sugar deposition in secondary cell walls enhances monomeric sugar release after low temperature ionic liquid pretreatment

  Rate of enzymatic saccharification 10 % loading at 72 h
   Rate glucose Rate xylose
(mg/L/min) (mg/L/min)
WT Untreated 43 ± 2 51 ± 10
  10 % 70 °C, 5 h 86 ± 16 n.d.
  10 % 140 °C, 3 h 196 ± 7 96 ± 42
LLL Untreated 30 ± 0.5 41 ± 7
  10 % 70 °C, 5 h 41 ± 7* 46 ± 1**
  10 % 140 °C, 3 h 255 ± 10* 154 ± 33
LLHPL1 Untreated 54 ± 7 58 ± 20
  10 % 70 °C, 5 h 52 ± 8* 68 ± 9**
  10 % 140 °C, 3 h 271 ± 13* 221 ± 16*
LLHPL2 Untreated 19 ± 10 41 ± 20
  10 % 70 °C, 5 h 40 ± 16** 62 ± 13**
  10 % 140 °C, 3 h 221 ± 16 146 ± 50
  1. Values presented ±SD. There were significant differences between Arabidopsis lines for both the initial glucose and xylose rates for solids pretreated at 70 °C (glucose, F(3,12) = 8.8, P < 0.01 and xylose, F(3,12) = 43.6, P < 0.0001), and solids pretreated at 140 °C (glucose, F(3,12) = 7.35, P < 0.05 and xylose, F(3,12) = 5.66, P < 0.05). There was no significant difference of initial rate of xylose release between the groups in untreated (xylose, F(3,12) = 0.62, P = 0.62), but there was a significant difference between groups for initial rate of glucose release (glucose, F(3,12) = 11.22, P < 0.05). ANOVA with a Tukey’s HSD post-hoc test was used to determine overall statistics, and results of the comparison to WT from the post-hoc test are shown in the table
  2. n.d. not detectable
  3. * P < 0.05; ** P < 0.01