Restricting lignin and enhancing sugar deposition in secondary cell walls enhances monomeric sugar release after low temperature ionic liquid pretreatment

Table 5 Rate of enzymatic saccharification as calculated by release during the first 30 min of enzymatic hydrolysis with both the cellulase and hemicellulase mixtures CTec2 and HTec2

	Rate of enzymatic saccharification 10 % loading at 72 h
		Rate glucose	Rate xylose
		(mg/L/min)	(mg/L/min)
WT	Untreated	43 ± 2	51 ± 10
	10 % 70 °C, 5 h	86 ± 16	n.d.
	10 % 140 °C, 3 h	196 ± 7	96 ± 42
LLL	Untreated	30 ± 0.5	41 ± 7
	10 % 70 °C, 5 h	41 ± 7^*	46 ± 1^**
	10 % 140 °C, 3 h	255 ± 10^*	154 ± 33
LLHPL1	Untreated	54 ± 7	58 ± 20
	10 % 70 °C, 5 h	52 ± 8^*	68 ± 9^**
	10 % 140 °C, 3 h	271 ± 13^*	221 ± 16^*
LLHPL2	Untreated	19 ± 10	41 ± 20
	10 % 70 °C, 5 h	40 ± 16^**	62 ± 13^**
	10 % 140 °C, 3 h	221 ± 16	146 ± 50

Values presented ±SD. There were significant differences between Arabidopsis lines for both the initial glucose and xylose rates for solids pretreated at 70 °C (glucose, F_(3,12) = 8.8, P < 0.01 and xylose, F_(3,12) = 43.6, P < 0.0001), and solids pretreated at 140 °C (glucose, F_(3,12) = 7.35, P < 0.05 and xylose, F_(3,12) = 5.66, P < 0.05). There was no significant difference of initial rate of xylose release between the groups in untreated (xylose, F_(3,12) = 0.62, P = 0.62), but there was a significant difference between groups for initial rate of glucose release (glucose, F_(3,12) = 11.22, P < 0.05). ANOVA with a Tukey’s HSD post-hoc test was used to determine overall statistics, and results of the comparison to WT from the post-hoc test are shown in the table
n.d. not detectable
^* P < 0.05; ^** P < 0.01

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