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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Development of a stress-induced mutagenesis module for autonomous adaptive evolution of Escherichia coli to improve its stress tolerance

Fig. 4

Improving n-butanol tolerance of E. coli by periodic adaptive evolution via the SIM module. a The scheme. b The n-butanol MIC after each cycle of evolution. The strain SMB07/pML/pTL16 equipped with the SIM module and the control strain FC40/pACYC184/pTAD without the module were compared. c-f Growth curves of the evolved strain SMB705/pML/pTL16 and its parent strain SMB07/pML/pTL16 at 37 °C in various media: c LB medium with various concentrations of n-butanol; d LB medium with 50 or 60 g/L NaCl; e LB medium at pH 4.8, adjusted by lactic acid; f M9 minimal medium containing 0.2 % glucose. For all the media, 100 μg/mL ampicillin, 25 μg/mL tetracycline, and 500 ng/mL aTc were added. All the OD600 values were detected using 200 μL of culture in 96-well microplates. Three independent cultures were prepared for each condition

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