Table 2 List of P. ellipsoidea and E. coli strains used in this study
From: Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea
Strains | Genotype | Source or reference |
|---|---|---|
P. ellipsoidea | Â | Â |
Obi | Wild-type | [13] |
M2 | Carry a 27-base deletion within the OPRTase domain of UMPS | This study |
M3 | Carry a 29-base insertion within the ODCase domain of UMPS | This study |
M4 | Carry a 192-base deletion within the ODCase domain of UMPS | This study |
TAL-1 | Carry two chromosomally integrated DNA fragments encoding TALEN left and right arms | This study |
E. coli | Â | Â |
DH5α | F− endA1, glnV44, thi-1, recA1, relA1, gyrA96, deoR, nupG, Φ80dlacZΔM15, Δ(lacZYA-argF)U169, hsdR17 (rK− mK+), λ− | TOYOBO |
JD24489 | W3110 (typeA) lacIq, lacZΔM15, galK2, galK22, ∆pyrE::kan | National Institute of Genetics |
JW1273 | ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), λ−, ∆pyrF789::kan, rph-1, ∆ rhaD-rhaB)568, hsdR514 (rK− mK+) | National Institute of Genetics |