Skip to main content

Table 2 List of P. ellipsoidea and E. coli strains used in this study

From: Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea

Strains Genotype Source or reference
P. ellipsoidea   
Obi Wild-type [13]
M2 Carry a 27-base deletion within the OPRTase domain of UMPS This study
M3 Carry a 29-base insertion within the ODCase domain of UMPS This study
M4 Carry a 192-base deletion within the ODCase domain of UMPS This study
TAL-1 Carry two chromosomally integrated DNA fragments encoding TALEN left and right arms This study
E. coli   
DH5α F endA1, glnV44, thi-1, recA1, relA1, gyrA96, deoR, nupG, Φ80dlacZΔM15, Δ(lacZYA-argF)U169, hsdR17 (rK mK+), λ TOYOBO
JD24489 W3110 (typeA) lacIq, lacZΔM15, galK2, galK22, ∆pyrE::kan National Institute of Genetics
JW1273 ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), λ, ∆pyrF789::kan, rph-1, ∆ rhaD-rhaB)568, hsdR514 (rK mK+) National Institute of Genetics