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Table 2 List of P. ellipsoidea and E. coli strains used in this study

From: Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea

Strains

Genotype

Source or reference

P. ellipsoidea

  

Obi

Wild-type

[13]

M2

Carry a 27-base deletion within the OPRTase domain of UMPS

This study

M3

Carry a 29-base insertion within the ODCase domain of UMPS

This study

M4

Carry a 192-base deletion within the ODCase domain of UMPS

This study

TAL-1

Carry two chromosomally integrated DNA fragments encoding TALEN left and right arms

This study

E. coli

  

DH5α

F endA1, glnV44, thi-1, recA1, relA1, gyrA96, deoR, nupG, Φ80dlacZΔM15, Δ(lacZYA-argF)U169, hsdR17 (rK mK+), λ

TOYOBO

JD24489

W3110 (typeA) lacIq, lacZΔM15, galK2, galK22, ∆pyrE::kan

National Institute of Genetics

JW1273

∆(araD-araB)567, ∆lacZ4787(::rrnB-3), λ, ∆pyrF789::kan, rph-1, ∆ rhaD-rhaB)568, hsdR514 (rK mK+)

National Institute of Genetics