From: Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea
Primer name | Sequence (5â€² to 3â€²) | Target |
---|---|---|
Umps_F | CCACAAAACCCATTGCCTCAACA | 5â€²-end of UMPS |
Umps_R | TCCGTGCTGTCTTCCAGGTCTT | 3â€²-end of UMPS |
Umps_cDNAF | CCAAGCTTTGCATGGCATTCAACAAGGTGAA | 5â€²-end of UMPS cDNA |
Umps_cDNAR | GGAATTCCCAACCTCATTTGAGAGGCAAT | 3â€²-end of UMPS cDNA |
Umps_CDRF | CACCCATGGCAACTTCAACTCCCTCAGT | 5â€²-end of UMPS CDR |
Umps_CDRR | CACAAGCTTCATGCCAGCGTGGCCTCATA | 3â€²-end of UMPS CDR |
Ptub_F | CCAAGCTTTGCATGGCATTCAACAAGGTGAA | TUBULIN1 promoter |
Ptub_R_Umps5â€² | GAGTTGAAGTTGCCATCTTGAACGACTTCCCTCGACAA | 5â€²-end of UMPS CDR and 3â€²-end of TUBULIN promoter |
Umps3â€²_Tact_F | GCCACGCTGGCATGAGGTGAGCCTGAAACAGCTTTCTG | 3â€²-end of UMPS CDR and 5â€²-end of ACT1 terminator |
Tact_R | TCGGTACCGGTTGTTGAGTTGAGCACTGGAC | 3â€²-end of ACT1 terminator |
Tact_R2 | CCCCAGCTGCTACTGCTATC | A middle part of ACT1 terminator |
Prbc_F | CTCAATCGGCCGATTTCATGCATGA | 5â€²-end of RBCS promoter |
Trbc_R | GTGCTGGTCTGTTTCCATGCAGTCAT | 3â€²-end of RBCS terminator |
Ptub3â€²_Umps_CDRF | GGGAAGTCGTTCAAGATGGCAACTTCAACTCCCTCAG | 3â€²-end of TUBULIN promoter and 5â€²-end of UMPS CDR |
Umps_CDRR_Tact5â€² | TGTTTCAGGCTCACCTCATGCCAGCGTGGCCTCATA | 5â€²-end of ACT1 terminator and 3â€²-end of UMPS CDR |
G418F | GATCGGCCATTGAACAAGAT | nptII |
G418R | GCGATACCGTAAAGCACGAG | nptII |
Umps_QRT_F | TCTTCGACAAGTGGGATGATGG | Position 908â€“930 of UMPS CDR |
Umps_QRT_R | TGTTACCGATGTCCGCAAAC | Position 993â€“1012 of UMPS CDR |
18SrRNA_QRT_F | GGATCAATTGGAGGGCAAGT | Position 627â€“646 of 18S rRNA |
18SrRNA_QRT_R | GCCCGAAATCCAACTACGAG | Position 713â€“732 of 18S rRNA |