Table 3 List of PCR primers used in this study
From: Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea
Primer name | Sequence (5′ to 3′) | Target |
|---|---|---|
Umps_F | CCACAAAACCCATTGCCTCAACA | 5′-end of UMPS |
Umps_R | TCCGTGCTGTCTTCCAGGTCTT | 3′-end of UMPS |
Umps_cDNAF | CCAAGCTTTGCATGGCATTCAACAAGGTGAA | 5′-end of UMPS cDNA |
Umps_cDNAR | GGAATTCCCAACCTCATTTGAGAGGCAAT | 3′-end of UMPS cDNA |
Umps_CDRF | CACCCATGGCAACTTCAACTCCCTCAGT | 5′-end of UMPS CDR |
Umps_CDRR | CACAAGCTTCATGCCAGCGTGGCCTCATA | 3′-end of UMPS CDR |
Ptub_F | CCAAGCTTTGCATGGCATTCAACAAGGTGAA | TUBULIN1 promoter |
Ptub_R_Umps5′ | GAGTTGAAGTTGCCATCTTGAACGACTTCCCTCGACAA | 5′-end of UMPS CDR and 3′-end of TUBULIN promoter |
Umps3′_Tact_F | GCCACGCTGGCATGAGGTGAGCCTGAAACAGCTTTCTG | 3′-end of UMPS CDR and 5′-end of ACT1 terminator |
Tact_R | TCGGTACCGGTTGTTGAGTTGAGCACTGGAC | 3′-end of ACT1 terminator |
Tact_R2 | CCCCAGCTGCTACTGCTATC | A middle part of ACT1 terminator |
Prbc_F | CTCAATCGGCCGATTTCATGCATGA | 5′-end of RBCS promoter |
Trbc_R | GTGCTGGTCTGTTTCCATGCAGTCAT | 3′-end of RBCS terminator |
Ptub3′_Umps_CDRF | GGGAAGTCGTTCAAGATGGCAACTTCAACTCCCTCAG | 3′-end of TUBULIN promoter and 5′-end of UMPS CDR |
Umps_CDRR_Tact5′ | TGTTTCAGGCTCACCTCATGCCAGCGTGGCCTCATA | 5′-end of ACT1 terminator and 3′-end of UMPS CDR |
G418F | GATCGGCCATTGAACAAGAT | nptII |
G418R | GCGATACCGTAAAGCACGAG | nptII |
Umps_QRT_F | TCTTCGACAAGTGGGATGATGG | Position 908–930 of UMPS CDR |
Umps_QRT_R | TGTTACCGATGTCCGCAAAC | Position 993–1012 of UMPS CDR |
18SrRNA_QRT_F | GGATCAATTGGAGGGCAAGT | Position 627–646 of 18S rRNA |
18SrRNA_QRT_R | GCCCGAAATCCAACTACGAG | Position 713–732 of 18S rRNA |