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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Butanol production in S. cerevisiae via a synthetic ABE pathway is enhanced by specific metabolic engineering and butanol resistance

Fig. 2

Deletion of the ADH1 gene improves butanol production in S. cerevisiae. a A schematic diagram of how the pathway of glucose fermentation to ethanol is connected to the added butanol production pathway. The step affected by the ADH1 deletion is highlighted and the balance of reducing equivalent in the form of NADH or NADPH through the pathway is detailed. b The strategy for ADH1 deletion and screening of candidates on Actinomycin A plates. c PCR analysis on genomic DNAs derived from either the adh1Δ strains or their parent. The primers used and resulting PCR products are detailed above the gels. d and e Graphs depicting the level of ethanol or butanol produced from the adh1Δ strain or from strains bearing the five butanol production genes either alone (BR + 5 g) or in combination with adh1Δ (BR adh1Δ +5 g) over a 21-day anaerobic fermentation. Error bars are ± SEM from five biological repeats

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