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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Butanol production in S. cerevisiae via a synthetic ABE pathway is enhanced by specific metabolic engineering and butanol resistance

Fig. 5

Expression of an acetyl-CoA driving force in the context of a butanol resistant allele of GCD1 further improves butanol levels. a A schematic diagram of the yeast genomic integration cassette designed to drive high level ALD6 ACS2 expression. b Western blotting using an anti-Flag antibody on protein samples from strains where the ALD6 ACS2 (A6A2) cassette has been integrated relative to the parent strains bearing the five butanol production enzymes and controls. Protein products are labelled to the right of the gel image. A blot probed with an anti-Pab1p antibody provides a loading control (lower panel). c and d Graphs depicting the level of ethanol or butanol produced from adh1Δ mutant strains bearing five butanol production genes (+5 g) and the ALD6 ACS2 expression cassette (A6A2). Data from 21-day anaerobic fermentations for both BR (GCD1-P180) and BS (GCD1-S180) derived strains are shown. Error bars are ± SEM from six biological repeats

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