Immunogold localization of xylans by LM10 in Sf and Par of M. × giganteus. In raw materials, the majority of xylans were present in the Sw layer of Sf as a nearly continuous matrix rather than in the Ccml and Cml areas (a), while the Par walls showed a uniform distribution (b). In 160°C-treated samples, the xylans were clearly less abundant in the regions of Ccml and the adjacent outer Sw of Sf, but there was a retention near the middle Sw of corresponding cells (c, asterisk). The golden particles in the Par were much more sparse after acid treatment (d). In 170°C-treated samples, only infrequently were any xylans detected on the surface of Sf and Par (e, f). Ccml compound cell corner; Cml compound middle lamella; Sw secondary wall.