Table 2 Characteristics of lipid production in continuous cultures of Y. lipolytica JMY4086 grown in crude glycerol; SCFB and chemostat culturing were used
Culture method | Glycerol feeding (g/L) | Biomass (gCDW/L) | Residual glycerol (g/L) | Lipid (g/L) | Citric acid (g/L) | Q L (g/L/h) | Y L/gly (g/g) | Y L/X (g/g) |
|---|---|---|---|---|---|---|---|---|
SCFB | 100 | 32.2 ± 1.2 | nd | 6.5 ± 1.1 | nd | 0.09 ± 0.01 | 0.13 | 0.2 |
SCFB | 200 | 39.1 ± 6.5 | nd | 14.8 ± 3.9 | 0.26 ± 0.4 | 0.26 ± 0.01 | 0.09 | 0.37 |
SCFB | 250 | 49.1 ± 9.6 | nd | 22.6 ± 2.1 | 0.26 ± 0.1 | 0.31 ± 0.01 | 0.08 | 0.46 |
SCFB | 350 | 64.7 ± 2.9 | nd | 24.1 ± 0.9 | 24.3 ± 1.6 | 0.26 ± 0.01 | 0.06 | 0.36 |
SCFB | 450 | 67.4 ± 1.4 | 0.50 ± 0.05 | 23.7 ± 1.0 | 60 ± 1.1 | 0.25 ± 0.01 | 0.05 | 0.35 |
Chemostat | 250 | 59.8 ± 0.1 | nd | 24.2 ± 0.1 | 50.2 ± 0.2 | 0.43 ± 0.01 | 0.10 | 0.4 |