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Table 1 Strain and plasmids used in this study

From: Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification

Strains Strain and genotype/phenotype Source
Escherichia coli
 JW334 DH5α containing pDCW171 (ApramycinR) This study
Caldicellulosiruptor bescii
 JWCB001 Wild-type/(ura+/5-FOAS) DSMZa
 JWCB018 ΔpyrFA ldh::ISCbe4 ΔcbeI/(ura/5-FOAR) [22, 47]
 JWCB044 ΔpyrFA ldh::ISCbe4 ΔcbeI :: PS-layerTeth39_1597/(ura/5-FOAR) This study
Plasmids
 pET30a-Teth39_1597 Source of Teth39_1597 open reading frame [11]
 pDCW142 Intermediate vector (ApramycinR) [19]
 pDCW171 Integration vector containing PS-layerTeth39_1597 (ApramycinR) This study
  1. aDeutsche Sammlung von Mikroorganismen und Zellkulturen.