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Table 1 Strain and plasmids used in this study

From: Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification

Strains

Strain and genotype/phenotype

Source

Escherichia coli

 JW334

DH5α containing pDCW171 (ApramycinR)

This study

Caldicellulosiruptor bescii

 JWCB001

Wild-type/(ura+/5-FOAS)

DSMZa

 JWCB018

ΔpyrFA ldh::ISCbe4 ΔcbeI/(ura−/5-FOAR)

[22, 47]

 JWCB044

ΔpyrFA ldh::ISCbe4 ΔcbeI :: PS-layerTeth39_1597/(ura−/5-FOAR)

This study

Plasmids

 pET30a-Teth39_1597

Source of Teth39_1597 open reading frame

[11]

 pDCW142

Intermediate vector (ApramycinR)

[19]

 pDCW171

Integration vector containing PS-layerTeth39_1597 (ApramycinR)

This study

  1. aDeutsche Sammlung von Mikroorganismen und Zellkulturen.