Strains | Strain and genotype/phenotype | Source |
---|---|---|
Escherichia coli | ||
 JW334 | DH5α containing pDCW171 (ApramycinR) | This study |
Caldicellulosiruptor bescii | ||
 JWCB001 | Wild-type/(ura+/5-FOAS) | DSMZa |
 JWCB018 | ΔpyrFA ldh::ISCbe4 ΔcbeI/(ura−/5-FOAR) | |
 JWCB044 | ΔpyrFA ldh::ISCbe4 ΔcbeI :: PS-layerTeth39_1597/(ura−/5-FOAR) | This study |
Plasmids | ||
 pET30a-Teth39_1597 | Source of Teth39_1597 open reading frame | [11] |
 pDCW142 | Intermediate vector (ApramycinR) | [19] |
 pDCW171 | Integration vector containing PS-layerTeth39_1597 (ApramycinR) | This study |