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Table 2 Mass balance of 15 mM furfural or 15 mM 5-HMF containing C. bescii batch fermentations

From: Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification

  Concentrations (mM) Carbon Recovery (%)a Concentrations (mM) O/Rd
Cellobiose consumed Glucose Acetate Lactate CO2 Biomass H2 Furfuryl alcoholb 5-HMF alcoholc
15 mM Furfural
 JWCB001 6.5 ± 0.6 7.2 ± 0.6 7.3 ± 0.6 3.4 ± 0.3 7.7 ± 0.5 0.4 ± 0.0 12.9 ± 0.9 98.51 3.6 ± 0.8   0.90
 JWCB018 5.3 ± 0.3 4.6 ± 0.3 10.5 ± 0.6 ND 10.8 ± 0.8 0.4 ± 0.1 15.5 ± 1.2 95.40 3.2 ± 06   1.11
 JWCB044 6.7 ± 0.4 6.0 ± 0.4 14.6 ± 1.0 ND 15.0 ± 0.7 0.5 ± 0.0 18.7 ± 0.7 102.82 13.1 ± 0.6   0.92
15 mM 5-HMF
 JWCB001 10.1 ± 0.5 7.3 ± 0.5 14.4 ± 0.5 9.8 ± 0.9 15.6 ± 0.4 1.2 ± 0.1 24.5 ± 2.5 100.95   3.4 ± 0.4 1.03
 JWCB018 9.5 ± 0.3 6.7 ± 0.6 17.8 ± 1.2 ND 18.8 ± 1.5 1.2 ± 0.1 31.9 ± 1.8 87.39   3.9 ± 0.7 1.01
 JWCB044 10.1 ± 0.2 7.7 ± 0.7 18.2 ± 1.5 ND 19.4 ± 0.4 1.2 ± 0.2 30.9 ± 1.9 87.57   13.6 ± 0.0 0.84
  1. Metabolite concentrations were determined at the end of 72 h batch fermentations.
  2. ND not detected.
  3. aCarbon recovery is the ratio of carbon compounds produced divided by the carbon compounds consumed and is expressed as a percentage.
  4. bFurfuryl alcohol was not reliably quantifiable via HPLC analyses. Values represent the reduction in furfural from the culture medium and assume 100% reduction to furfuryl alcohol.
  5. cAmount of 5-HMF removed from the culture medium was 3.8 ± 0.5 mM (JWCB001), 4.5 ± 0.7 mM (JWCB018) and 14.9 ± 0.0 mM (JWCB044).
  6. dRatio of oxidized end-products to reduced end-products.