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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes

Fig. 3

Western blot detection of the expressed β-glucosidases a M, molecular weight standards; lane 1, intracellular Bgl1, b lane 1, extracellular Bgl1-His6, c lane 1, intracellular Bgl2-His6, d lane 1, extracellular Bgl2-His6, and SDS-PAGE analysis of the purified β-glucosidases from Y. lipolytica JMY1212 transformants e lane 1, purified Bgl1-His6, and f lane 1, purified Bgl2; lane 2, endo-H treated Bgl2 (The lower band in lane 2 represents the expected size of Endo-H).

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