Western blot detection of the expressed β-glucosidases a M, molecular weight standards; lane 1, intracellular Bgl1, b
lane 1, extracellular Bgl1-His6, c
lane 1, intracellular Bgl2-His6, d
lane 1, extracellular Bgl2-His6, and SDS-PAGE analysis of the purified β-glucosidases from Y. lipolytica JMY1212 transformants e
lane 1, purified Bgl1-His6, and f
lane 1, purified Bgl2; lane 2, endo-H treated Bgl2 (The lower band in lane 2 represents the expected size of Endo-H).