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Table 1 Distribution of β-glucosidase activity in recombinant strains ZetaB 1 and ZetaB 2

From: Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes

Fraction Relative enzyme activitya
Bgl1 (%) Bgl2 (%)
Total 100 100
Growth medium 2.3 ± 0.4 79.6 ± 1.2
Periplasm 60.7 ± 1.0 25.8 ± 1.0
Cytoplasm 30.0 ± 0.5 4.8 ± 0.8
Membrane 8.1 ± 0.7 3.7 ± 0.3
  1. ±, the standard deviation.
  2. aTriplicate experiments. Activity was assayed with pNP-βGlc.