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Table 1 Distribution of β-glucosidase activity in recombinant strains ZetaB 1 and ZetaB 2

From: Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes

Fraction

Relative enzyme activitya

Bgl1 (%)

Bgl2 (%)

Total

100

100

Growth medium

2.3 ± 0.4

79.6 ± 1.2

Periplasm

60.7 ± 1.0

25.8 ± 1.0

Cytoplasm

30.0 ± 0.5

4.8 ± 0.8

Membrane

8.1 ± 0.7

3.7 ± 0.3

  1. ±, the standard deviation.
  2. aTriplicate experiments. Activity was assayed with pNP-βGlc.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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