Table 2 Purification of intracellular Bgl1-His6 and extracellular Bgl2 produced by Y. lipolytica overexpressing strains
Enzyme and purification method | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Fold purification | Yield (%) recovery |
|---|---|---|---|---|---|
Bgl1-His6 | |||||
Filtrate | 169.7 | 543.0 | – | – | 100 |
TALON His-taga | 0.9 | 92.5 | 102.8 | 32.1 | 17.0 |
Bgl2 | |||||
Culture supernatant | 2302.5 | 530.2 | – | – | 100 |
Ultra filtration | 1986.4 | 510.5 | – | 1.1 | 96.3 |
Ion exchange | 235.3 | 478.5 | – | 7.7 | 90.2 |
Gel filtration | 1.8 | 46.4 | 25.8 | 112.2 | 8.8 |