Skip to main content

Table 2 Purification of intracellular Bgl1-His6 and extracellular Bgl2 produced by Y. lipolytica overexpressing strains

From: Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes

Enzyme and purification method Total protein (mg) Total activity (U) Specific activity (U/mg) Fold purification Yield (%) recovery
Bgl1-His6
 Filtrate 169.7 543.0 100
 TALON His-taga 0.9 92.5 102.8 32.1 17.0
Bgl2
 Culture supernatant 2302.5 530.2 100
 Ultra filtration 1986.4 510.5 1.1 96.3
 Ion exchange 235.3 478.5 7.7 90.2
 Gel filtration 1.8 46.4 25.8 112.2 8.8
  1. aSpecific activity was tested on pNP-βGlc.