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Table 3 Kinetic parameters of Y. lipolytica Bgls for various glycoside-substrates

From: Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes

Substrate Linkage Bgl1 Bgl2
K M (mM) k cat (s−1) k cat /K M (mM−1 s−1) K M (mM) k cat (s−1) k cat /K M (mM−1 s−1)
Cellobiose Glc × 2, β-l, 4 0.26 21.1 81.1 0.79 5.1 6.5
Cellotriose Glc × 3, β-1, 4 0.43 20.5 47.7 0.99 9.5 9.6
Cellotetraose Glc × 4, β-1, 4 1.89 30.9 16.3 1.86 20.6 11
Cellopentaose Glc × 5, β-1, 4 2.18 29.5 13.5 2.24 27.5 12.3
Cellohexaose Glc × 6, β-1, 4 3.01 31.5 10.5 2.37 30.5 12.9
Sophorose Glc × 2, β-1, 2 2.25 28.4 14.8 2.4 41.2 17.2
Laminaribiose Glc × 2, β-1, 3 0.68 75.6 110.7 0.89 211.1 237.2
Gentiobiose Glc × 2, β-1, 6 1.16 43.6 37.6 1.84 186.5 101.4
Methylglucoside C = l 15 15 1 6.23 34.1 5.5
Octylglucoside C = 8 0.86 32.8 38.1 1.3 111.1 85.2
  1. The mean values of three independent experiments are shown and the standard deviation is below 10%. Hydrolytic activities for the substrate were determined from the amount of released glucose and the kinetic parameters were calculated as described in “Methods”.