Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes

Table 3 Kinetic parameters of Y. lipolytica Bgls for various glycoside-substrates

Substrate	Linkage	Bgl1			Bgl2
Substrate	Linkage	K _M (mM)	k _cat (s⁻¹)	k _cat/K _M (mM⁻¹ s⁻¹)	K _M (mM)	k _cat (s⁻¹)	k _cat/K _M (mM⁻¹ s⁻¹)
Cellobiose	Glc × 2, β-l, 4	0.26	21.1	81.1	0.79	5.1	6.5
Cellotriose	Glc × 3, β-1, 4	0.43	20.5	47.7	0.99	9.5	9.6
Cellotetraose	Glc × 4, β-1, 4	1.89	30.9	16.3	1.86	20.6	11
Cellopentaose	Glc × 5, β-1, 4	2.18	29.5	13.5	2.24	27.5	12.3
Cellohexaose	Glc × 6, β-1, 4	3.01	31.5	10.5	2.37	30.5	12.9
Sophorose	Glc × 2, β-1, 2	2.25	28.4	14.8	2.4	41.2	17.2
Laminaribiose	Glc × 2, β-1, 3	0.68	75.6	110.7	0.89	211.1	237.2
Gentiobiose	Glc × 2, β-1, 6	1.16	43.6	37.6	1.84	186.5	101.4
Methylglucoside	C = l	15	15	1	6.23	34.1	5.5
Octylglucoside	C = 8	0.86	32.8	38.1	1.3	111.1	85.2

The mean values of three independent experiments are shown and the standard deviation is below 10%. Hydrolytic activities for the substrate were determined from the amount of released glucose and the kinetic parameters were calculated as described in “Methods”.

ISSN: 2731-3654