Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes

Table 7 The sequences of the oligonucleotide primers used in this study

Primer names	Sequence (5′–3′) restriction sites are italic/underlined	Restriction sites
FA1	CG^a GGATCCCGCGATGATCTTCTCTCTGCAACTACTAC	BamHI
RB1	CGCCTAGGCTACAAAGTGAAAGTCTCACATAGC	AvrII
FA2	CCCAAGCTTGGGTTTGGAGGGGGTGAAAAA	HindIII
RB2	CCCAAGCTTGGGCTAAAGACCTAACCAATTCTTAGTCT	HindIII
FA3	CGGGATCCCGCGATGATTGCAAAAATACCCC	BamHI
RB3	CGCCTAGGCTACTGGAGAGTAAAGGACTCG	AvrII
FA4	CGGGATCCCGCGATGCTCGCATTCGTCCTAC	BamHI
RB4	CGGGATCCCGCTACTTGAGAGTGAAGCTGGTG	BamHI
FA5	CGGGATCCCGCGATGGCTCCACCCCCGCCTCCT	BamHI
RB5	CGCCTAGGTTAAGCAATCGTGATGCGACCAAGG	AvrII
FA6	CGCCTAGGCGCGATGGAGGAATTATCGGAGGC	AvrII
RB6	CGCCTAGGCTACCGGCTGAACTTCTCTTC	AvrII
RB-His1	^b CGCCTAGGTTAATGATGGTGATGATGGTGGCTGCCGCGCGGCACCAGCCTAGGCAAAGTGAAAGTCTCA
RB-His2	CCCAAGCTTGGGTTAATGATGGTGATGATGGTGGCTGCCGCGCGGCACCAGCCTAGGAAGACCTAACCAATTCTTA
RB-His3	CGCCTAGGTTAATGATGGTGATGATGGTGGCTGCCGCGCGGCACCAGCCTAGGCTGGAGAGTAAAGGA
RB-His4	CGGGATCCCGTTAATGATGGTGATGATGGTGGCTGCCGCGCGGCACCAGCCTAGGCTTGAGAGTGAAGCT
RB-His5	CGCCTAGGTTAATGATGGTGATGATGGTGGCTGCCGCGCGGCACCAGCCTAGGAGCAATCGTGATGC
RB-His6	CGCCTAGGTTAATGATGGTGATGATGGTGGCTGCCGCGCGGCACCAGCCTAGGCTGAACTTCTCTTCC

ISSN: 2731-3654