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Table 1 Strains and plasmids used in this study

From: Alleviation of carbon catabolite repression in Enterobacter aerogenes for efficient utilization of sugarcane molasses for 2,3-butanediol production

Strains or plasmids

Genotype or relevant characteristics

Source or reference

Strain

 E. coli DH5α

 

Invitrogen

 E. aerogenes KCTC 2190

Wild type

Korean Collection for Type Culture

 EMY-01

E. aerogenes KCTC 2190 â–³ldhA

[27]

 EMY-68

E. aerogenes KCTC 2190 â–³ldhA â–³scrR

[19]

 EMY-69

E. aerogenes KCTC 2190 â–³ldhA â–³cra

This study

 EMY-70

E. aerogenes KCTC 2190 â–³ldhA â–³scrR â–³cra

This study

 EMY-70S

Plasmid-based scrAB overexpression strain of EMY-70 by introduction of pZS21MCS::scrAB

This study

 EMY-70SP

Plasmid-based ptsG overexpression strain of EMY-70S by introduction of pZA31MCS::ptsG

This study

Plasmid

 pKM208

lacI, λ Red + Gam-producing vector, tac_promoter, f1_ori, Amp R

Addgene

 pCP20

FLP recombinase-producing vector, cI857, pSC101 ori, Amp R, Cm R

[40]

 pKD4

FRT flanked resistance cassette involved vector, oriRγ, Km R

[40]

 pZA31MCS

E. coli—K. pneumoniae shuttle vector, PLtetO-1, p15A ori, CmR

Expressys

 pZS21MCS

E. coli—K. pneumoniae shuttle vector, PLtetO-1, pSC101 ori, Km R

Expressys

 pZA31MCS::ptsG

pZA31MCS derivative containing ptsG, PLtetO-1, p15A ori, CmR

This study

 pZS21MCS::scrAB

pZS21MCS derivative containing scrAB, PLtetO-1, pSC101 ori, Km R

This study