Strains or plasmids | Genotype or relevant characteristics | Source or reference |
---|---|---|
Strain | ||
E. coli DH5α | Invitrogen | |
E. aerogenes KCTC 2190 | Wild type | Korean Collection for Type Culture |
EMY-01 | E. aerogenes KCTC 2190 â–³ldhA | [27] |
EMY-68 | E. aerogenes KCTC 2190 â–³ldhA â–³scrR | [19] |
EMY-69 | E. aerogenes KCTC 2190 â–³ldhA â–³cra | This study |
EMY-70 | E. aerogenes KCTC 2190 â–³ldhA â–³scrR â–³cra | This study |
EMY-70S | Plasmid-based scrAB overexpression strain of EMY-70 by introduction of pZS21MCS::scrAB | This study |
EMY-70SP | Plasmid-based ptsG overexpression strain of EMY-70S by introduction of pZA31MCS::ptsG | This study |
Plasmid | ||
pKM208 | lacI, λ Red + Gam-producing vector, tac_promoter, f1_ori, Amp R | Addgene |
pCP20 | FLP recombinase-producing vector, cI857, pSC101 ori, Amp R, Cm R | [40] |
pKD4 | FRT flanked resistance cassette involved vector, oriRγ, Km R | [40] |
pZA31MCS | E. coli—K. pneumoniae shuttle vector, PLtetO-1, p15A ori, CmR | Expressys |
pZS21MCS | E. coli—K. pneumoniae shuttle vector, PLtetO-1, pSC101 ori, Km R | Expressys |
pZA31MCS::ptsG | pZA31MCS derivative containing ptsG, PLtetO-1, p15A ori, CmR | This study |
pZS21MCS::scrAB | pZS21MCS derivative containing scrAB, PLtetO-1, pSC101 ori, Km R | This study |