Table 1 Strains and plasmids used in this study
Strain | Descriptions | Source |
|---|---|---|
E. coli MG1655 | Wild type | Lab collection |
B. subtilis 168 | Wild type | Lab collection |
C. acetobutylicum ATCC 824 | Wild type | Lab collection |
L. lactis IL1403 | Wild type | Lab collection |
E. coli LA01 | MG1655ΔpflB | Lab collection |
E. coli LA02 | Amp, Cm; strain LA01 bearing pACYCLA09 and pTRCLA10 | This work |
E. coli LA03 | Amp, Cm; strain LA01 bearing pACYCLA09 and pTRCLA11 | This work |
E. coli LA04 | Amp, Cm; strain LA01 bearing pACYCLA09 and pTRCLA12 | This work |
E. coli LA05 | Amp, Cm; strain LA01 bearing pACYCLA09 and pTRCLA13 | This work |
E. coli LA06 | Amp, Cm; strain LA01 bearing pACYCLA09 and pTRCLA14 | This work |
E. coli LA07 | Amp, Cm; strain LA01 bearing pACYCLA09 and pTRCLA15 | This work |
E. coli LA08 | Amp, Cm; strain LA01 bearing pACYCLA09 and pTRCLA16 | This work |
E. coli LA09 | Amp, Cm; strain LA01 bearing pACYCLA09 and pTRCLA17 | This work |
Plasmid | ||
 pTRC99a | E.coli expression vector; Amp | Lab collection |
 pUC18 | E. coli expression vector; Amp | Lab collection |
 pACYC184 | E. coli expression vector; tetracycline and Cm | Lab collection |
 pTRCLA | E.coli expression vector, pBR322 ori, Amp | This work |
 pACYCLA | E.coli expression vector, pACYC ori, Cm | This work |
 pACYCLA09 | pACYC ori, Cm, Ptrc::alsS-ilvCD | This work |
 pTRCLA10 | pBR322 ori, Amp, Ptrc:: kivd-yqhD | This work |
 pTRCLA11 | pBR322 ori, Amp, Ptrc:: kivd-yqhD, PgapA::gapN | This work |
 pTRCLA12 | pBR322 ori, Amp, Ptrc:: kivd-yqhD, PgapA::gapC | This work |
 pTRCLA13 | pBR322 ori, Amp, Ptrc:: kivd-yqhD, BBa_J23105::gapN | This work |
 pTRCLA14 | pBR322 ori, Amp, Ptrc:: kivd-yqhD, BBa_J23106::gapN | This work |
 pTRCLA15 | pBR322 ori, Amp, Ptrc:: kivd-yqhD, BBa_J23118::gapN | This work |
 pTRCLA16 | pBR322 ori, Amp, Ptrc:: kivd-yqhD, BBa_J23102::gapN | This work |
 pTRCLA17 | pBR322 ori, Amp, Ptrc:: kivd-yqhD, BBa_J23100::gapN | This work |