Fig. 8

Effects of pretreatments followed by enzymatic hydrolysis in the NMR spectra. MultiCP spectra of sugarcane bagasse samples before and after chemical pretreatments (solid black lines) compared to samples that were also submitted to enzymatic hydrolysis after the chemical pretreatments (dashed red line). Curves were normalized considering the lignin signals at 120–160 ppm regions. The inset shows the percentage of cellulose removed by the enzymatic treatment obtained from the normalization scaling factors.