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Table 1 Insertion sites of allosteric clones selected from the semi-rational insertion library

From: Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase

Clone Position +xyl/–xyl ratioa
XynA parental 0.80 ± 0.04
1 Q262-263 1.20 ± 0.02
2 A209-210 1.19 ± 0.02
3 E304- 305 1.16 ± 0.01
4 E263-264 1.15 ± 0.02
5 N43-44 1.14 ± 0.02
6 D10-11 1.13 ± 0.01
7 E78-79 1.12 ± 0.02
8 I5-6 1.11 ± 0.02
9 D222-223 1.11 ± 0.03
  1. aXylanase activity in the supernatant (with xylose)/(without xylose). Data represent the mean ± SD of 3 repetitions (n = 3).