Skip to main content

Table 1 Insertion sites of allosteric clones selected from the semi-rational insertion library

From: Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase

Clone

Position

+xyl/–xyl ratioa

XynA parental

–

0.80 ± 0.04

1

Q262-263

1.20 ± 0.02

2

A209-210

1.19 ± 0.02

3

E304- 305

1.16 ± 0.01

4

E263-264

1.15 ± 0.02

5

N43-44

1.14 ± 0.02

6

D10-11

1.13 ± 0.01

7

E78-79

1.12 ± 0.02

8

I5-6

1.11 ± 0.02

9

D222-223

1.11 ± 0.03

  1. aXylanase activity in the supernatant (with xylose)/(without xylose). Data represent the mean ± SD of 3 repetitions (n = 3).