Fig. 5

Relative quantification of enzymatic activity of the extracellular fraction of C. bescii expressing E1 (Acel0614) on Avicel and carboxymethylcellulose. Carboxymethylcellulose (CMC) or Avicel was used as substrate at either 65 or 75 °C. a Activity of the extracellular fraction (25 µg/mL concentrated protein) on CMC from the parent strain JWCB018 ΔpyrFA ldh::ISCbe4 Δcbe1 (black), the E1 expression strain, JWCB052 ΔpyrFA ldh::ISCbe4 Δcbe1::P _S-layer acel0614 (E1) (grey), and no enzyme control (white). b Activity of the extracellular fraction (25 µg/mL of concentrated protein) on Avicel from the parent strain JWCB018 (black), the E1 expression strain, JWCB052 (grey), and no enzyme control (white).