Table 1 Strains and plasmids used in this work

C. bescii

 JWCB001

Wild type (ura ⁺/5-FOA^S)

DSMZ^a

 JWCB018

ΔpyrFA ldh::ISCbe4 Δcbe1 (ura ⁻/5-FOA^R)

[29]

 JWCB052

ΔpyrFA ldh::ISCbe4 Δcbe1::P_S-layer acel0614 (E1) ^b (ura ^-/5-FOA^R)

This study

Escherichia coli

 JW314

DH5α containing pDCW144 (Apramycin^R)

[27]

 JW336

DH5α containing pDCW174 (Apramycin^R)

This study

 JW337

DH5α containing pDCW175 (Apramycin^R)

This study

Plasmids

 pDCW144

Integrational vector for Caldicellulosiruptor bescii (Apramycin^R)

[27]

 pDCW174

Integrational vector containing signal peptide of CelA for Caldicellulosiruptor bescii (Apramycin^R)

This study

 pDCW175

Integrational vector containing the P_S-layer acel0614 (E1) ^b expression cassette (Apramycin^R)

This study