C. bescii
|
JWCB001
|
Wild type (ura
+/5-FOAS)
|
DSMZa
|
JWCB018
|
ΔpyrFA ldh::ISCbe4 Δcbe1 (ura
−/5-FOAR)
|
[29]
|
JWCB052
|
ΔpyrFA ldh::ISCbe4 Δcbe1::PS-layer
acel0614 (E1)
b (ura
-/5-FOAR)
|
This study
|
Escherichia coli
|
JW314
|
DH5α containing pDCW144 (ApramycinR)
|
[27]
|
JW336
|
DH5α containing pDCW174 (ApramycinR)
|
This study
|
JW337
|
DH5α containing pDCW175 (ApramycinR)
|
This study
|
Plasmids
|
pDCW144
|
Integrational vector for Caldicellulosiruptor bescii (ApramycinR)
|
[27]
|
pDCW174
|
Integrational vector containing signal peptide of CelA for Caldicellulosiruptor bescii (ApramycinR)
|
This study
|
pDCW175
|
Integrational vector containing the PS-layer
acel0614 (E1)
b expression cassette (ApramycinR)
|
This study
|