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Table 1 Strains and plasmids used in this work

From: Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose

Strains/plasmids Strain and genotype/phenotype References
C. bescii
 JWCB001 Wild type (ura +/5-FOAS) DSMZa
 JWCB018 ΔpyrFA ldh::ISCbe4 Δcbe1 (ura /5-FOAR) [29]
 JWCB052 ΔpyrFA ldh::ISCbe4 Δcbe1::PS-layer acel0614 (E1) b (ura -/5-FOAR) This study
Escherichia coli
 JW314 DH5α containing pDCW144 (ApramycinR) [27]
 JW336 DH5α containing pDCW174 (ApramycinR) This study
 JW337 DH5α containing pDCW175 (ApramycinR) This study
Plasmids
 pDCW144 Integrational vector for Caldicellulosiruptor bescii (ApramycinR) [27]
 pDCW174 Integrational vector containing signal peptide of CelA for Caldicellulosiruptor bescii (ApramycinR) This study
 pDCW175 Integrational vector containing the PS-layer acel0614 (E1) b expression cassette (ApramycinR) This study
  1. a German Collection of Microorganisms and Cell Cultures.
  2. b acel0614 (E1) [glycoside hydrolase (family 5) with cellulose-binding (family 2) derived from Acidothermus cellulolyticus 11B].