Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose

Table 1 Strains and plasmids used in this work

Strains/plasmids	Strain and genotype/phenotype	References
C. bescii
JWCB001	Wild type (ura ⁺/5-FOA^S)	DSMZ^a
JWCB018	ΔpyrFA ldh::ISCbe4 Δcbe1 (ura ⁻/5-FOA^R)	[29]
JWCB052	ΔpyrFA ldh::ISCbe4 Δcbe1::P_S-layer acel0614 (E1) ^b (ura ^-/5-FOA^R)	This study
Escherichia coli
JW314	DH5α containing pDCW144 (Apramycin^R)	[27]
JW336	DH5α containing pDCW174 (Apramycin^R)	This study
JW337	DH5α containing pDCW175 (Apramycin^R)	This study
Plasmids
pDCW144	Integrational vector for Caldicellulosiruptor bescii (Apramycin^R)	[27]
pDCW174	Integrational vector containing signal peptide of CelA for Caldicellulosiruptor bescii (Apramycin^R)	This study
pDCW175	Integrational vector containing the P_S-layer acel0614 (E1) ^b expression cassette (Apramycin^R)	This study

^a German Collection of Microorganisms and Cell Cultures.
^b acel0614 (E1) [glycoside hydrolase (family 5) with cellulose-binding (family 2) derived from Acidothermus cellulolyticus 11B].

ISSN: 2731-3654