Fig. 2
Secretion phenotypes are not limited to cellulases. a Immunoblot analysis of total secreted protein from experiment outlined in Fig. 1. Equal volumes of culture supernatant were loaded and separated by electrophoresis on 10% polyacrylamide gels, then transferred to nitrocellulose membranes for antibody detection. Primary antibodies were directed against the following: CDH-1 (cellobiose dehydrogenase), GH5-1 (an endoglucanase), GH10-1 (an endoxylanase), GH61-5 (a polysaccharide monooxygenase), GH51-1 (an arabinofuranosidase), GH10-2 (an endoxylanase) and GLA-1 (glucoamylase). b Quantitation of detected proteins. For CDH-1, GH5-1, GH10-1 and GH61-5 values are given relative to WT in Avicel; for GH51-1 and GH10-2 values are given relative to WT in xylan; for GLA-1 values are given as relative to WT in sucrose.