Fig. 3

Secretion phenotypes are maintained on cellulosic substrates. a Total secreted protein. b, c Endoglucanase and xylanase activities, respectively. Conidia from wild type and the mutant strains were first inoculated into VMM (48 h) and the resulting biomass subsequently switched to Avicel, CMC, or Miscanthus (48 h). d Germination frequency. Conidia were cultured in sucrose (4 h) or Avicel broth (8 h) and the numbers of germinated conidia were evaluated by light microscopy. Statistical analyses for protein and enzyme activity levels were relative to WT within each condition (n = 3). Error bars indicate standard deviation.