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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Deletion of homologs of the SREBP pathway results in hyper-production of cellulases in Neurospora crassa and Trichoderma reesei

Fig. 6

Time course of expression levels of major cellulase genes in the Δtul-1 and Δsah-2 mutants relative to the parental wild-type strain under cellulolytic conditions. qRT-PCR experiments measuring gene expression levels from Avicel broth cultures inoculated with conidia from wild type or the Δtul-1 or Δsah-2 mutants and grown over a 120 h time course. Relative transcript levels of major cellulase genes cbh-1 (NCU07340), cbh-2 (NCU09680)and gh5-1 (NCU00762) were normalized to expression levels of actin (NCU04173). Primer sequences used are given in Additional file 5: Table S2. Data from n = 3 biological replicates; error bars indicate standard deviation.

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