Fig. 1

In vivo footprinting analysis of the Xpp1-binding region within the xyn2 promoter. T. reesei QM6aΔtmus53 and the xpp1 deletion strain were pre-cultured on glycerol and thereafter transferred to MA medium containing 50 mM d-glucose. DNA was methylated in vivo with DMS after 3 h incubation. Analysis of data was performed using ivFAST [22]. Significant differences in methylation intensities of individual purine nucleotides between the two strains are represented by squares (white, no difference; light gray, difference with ratios of more than 1.1 and less than 1.3; dark gray, differences with ratios of more than 1.3 and less 1.5; black, differences with ratios of more than 1.5). The sequence of the coding (upper lane) and non-coding (lower lane) strand from positions −241 to −220 of the xyn2 promoter is given. Bold letters indicate the previously described AGAA-boxes, underlined letters the palindromic sequence, and italic letters an atypical Xyr1-binding site [20].