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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Xpp1 regulates the expression of xylanases, but not of cellulases in Trichoderma reesei

Fig. 4

Influence of Xpp1 on transcript levels of xyn2 in T. reesei. a T. reesei QM6aΔtmus53 (blue bars) and the xpp1 deletion strain (green bars) were pre-cultured on glycerol and thereafter transferred to MA media without carbon source (NC), or containing 50 mM d-glucose (G), or 66 mM d-xylose (XO) and incubated for 3 h. Transcript levels refer to the reference sample (QM6aΔtmus53, NC). b T. reesei QM6aΔtmus53 (blue squares) and the xpp1 deletion strain (green squares) were grown in MA medium containing 1% (w/v) xylan. Samples were taken after 18, 24, 30, 36, 48, and 72 h of cultivation. Transcript levels of xyn2 were measured by qPCR using sar1 and act transcript levels for normalization and referred to the reference sample (QM6aΔtmus53, 18 h). Results are given as relative transcript ratios in logarithmic scale (Log). The values provided in the figures are means from three biological experiments. Error bars indicate standard deviations.

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