Fig. 3

Secretion of the various forms of Cel9A by the recombinant R. cellulolyticum strains. The various forms of Cel9A were detected by western blot using an antiserum raised against the C-terminal 6 His tag of the heterologous enzymes, on the supernatant of cellobiose-grown cultures. The lane labeled p0 designates the supernatant of the control strain carrying the empty expression vector p0. The lanes labeled pCel9A and pCel9Ac designate the supernatants of the recombinant strains carrying pCel9A and pCel9Ac, respectively. The lane labeled “Cel9A + Cel9Ac” corresponds to a mix of Cel9A and Cel9Ac purified from E. coli overproducing strains.