Fig. 5

Sub-cellular localization of the adaptor protein 3 (AP-3) complex in Neurospora crassa. Wild-type (WT) and ΔNcap3m strains were pre-grown in minimal medium with 2% (w/v) sucrose as the sole carbon source for 16 h and then switched to Avicel medium to elicit lignocellulase production for another 4 h (initial stage; a–d) or 48 h (logarithmic stage; e–h). Extreme tip regions of mycelia of the WT and ΔNcap3m were stained with 5 µg/mL of the membrane dye FM4-64 for 30 min to label membrane structures such as the Spitzenkörper or vacuoles (a, e). Locations of NcAP3m proteins were monitored by recording enhanced green fluorescent protein (EGFP) signal (b, f). Merged yellow fluorescence signal from FM4-64 and EGFP (c, g) are denoted by white arrows in the photos. Each scale bar represents 10 µm.