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Table 3 Fermentation profiles of T. saccharolyticum knockout strains

From: Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485

Strains

Additions to medium

Fermentation profilea

Unit: mmol in 50 mL culture

Name

Description

Consumed cellobiose

Residual cellobiose

Formate

Lactate

Acetate

Ethanol

Succinate

Pyruvate

Malate

Pellet carbon

Hydrogen

Carbon recovery (%)

Electron recovery (%)

LL1025

Wild type

None

0.70

0.00

0.01

0.28

0.71

1.18

0.01

0.00

0.00

0.80

1.68

90

94

LL1049

Ethanologenic strain

None

0.70

0.00

0.22

0.00

0.04

2.10

0.00

0.00

0.04

0.72

0.22

86

90

LL1139

LL1025 ΔpforA-1

None

0.07

0.63

0.15

0.04

0.05

0.09

0.00

0.00

0.00

0.18

0.01

98

99

LL1140

LL1025 ΔpforA‏-2

None

0.02

0.68

0.01

0.00

0.00

0.00

0.00

0.00

0.00

0.19

0.10

100

101

LL1141

Adapted from LL1139

None

0.37

0.34

0.28

0.67

0.06

0.29

0.00

0.06

0.03

0.27

0.02

91

92

LL1142

Adapted from LL1140

None

0.34

0.36

0.38

0.26

0.03

0.42

0.02

0.22

0.03

0.21

0.04

89

90

LL1155

LL1025 ΔpforD

None

0.70

0.00

0.02

0.39

0.72

1.20

0.01

0.00

0.00

0.72

1.65

91

94

LL1156

LL1025 ΔpforB

None

0.70

0.00

0.02

0.15

0.80

1.24

0.01

0.00

0.00

0.89

1.74

92

94

LL1157

LL1025 ΔpforF

None

0.70

0.00

0.00

0.38

0.76

1.19

0.00

0.00

0.00

0.73

1.64

91

94

LL1159

LL1049 ΔpforA

None

0.07

0.63

0.01

0.00

0.00

0.01

0.00

0.00

0.00

0.23

0.05

92

92

LL1164

LL1025 Δpfl–1

Formate

0.48

0.21

−0.02b

1.13

0.19

0.33

0.00

0.00

0.00

0.47

0.48

96

98

LL1170

LL1025 Δpf-2

Formate

0.69

0.00

−0.03b

0.24

0.81

1.22

0.00

0.00

0.00

0.92

0.69

92

95

LL1178

LL1025 ΔpforA; Δpfl

Formate and Acetate

0.48

0.25

−0.01c

1.69

−0.12c

0.08

0.00

0.00

0.01

0.31

0.00

94

96

  1. The amount of fermentation end products are reported in millimoles in a volume of 50 mL serum bottle. The amounts of Initial cellobiose were 0.70 mmol for all fermentations. Cultures were incubated for 72 h at 55 °C with an initial pH of 6.2 in MTC-6 medium.
  2. aThe standard deviations were less than 10 % for cellobiose, formate, lactate, acetate, ethanol, pyruvate, succinate and malate, which were measured by HPLC. For pellet carbon and hydrogen measurement, the standard deviation was less than 2 %. The calculated carbon recovery and electron recovery have a combined standard deviation less than 5 %.
  3. bTo improve the growth of LL1164, LL1170, 0.20 mmol formate was added into 50 mL MTC-6 medium. Negative values represent that a certain amount of sodium formate was consumed during fermentation.
  4. cLL1178 requires supplementation of both formate and acetate to grow in MTC-6 medium. 0.20 mmol sodium formate and 0.20 mmol sodium acetate were added into 50 mL MTC-6 medium. Negative values represent that a certain amount of sodium formate and sodium acetate was consumed during fermentation.